Description
Rubus ulmifolius Schoot (Rosaceae), known as wild blackberry, is a perennial shrubfound in wild
and cultivated habitats in Europe, Asia and North Africa[1]. Traditionally, R. ulmifoliusis considered
to be anticatarrhal, antiseptic, diuretic, anti-inflammatory, antioxidant, astringent, and
antispasmodic. Oral dosage forms are used for diarrhea, menstrual pain, menopause disorders,
liver diseases, aphtha, gingivitis, hypertension and diabetes [2].Different classes of bioactive
constituents are present in R. ulmifolius, including phenolic compounds. This study aimed to
determine the phenolic composition of different preparations (decoction and hydromethanolic
extract)of R. ulmifoliusflower buds. The analysis of phenolic compounds was carried out by
reversed-phase high performance liquid chromatography coupled to diode array detection and
mass spectrometry with electron spray ionization (HPLC-DAD-ESI/MS), in order to establish the
specific phenolic profile of each preparation. The phenolic profile of both preparations was
identical, varying only in the concentrations found. Twenty-three phenolic compounds were
identified in both samples, in which seven were identified as phenolic acids (di- and caffeolyqui nic,
p-coumaroylquinicand feruloylquinic acids),ten flavonoids (quercetin and kaempferol derivatives
and catechin), and five ellagitannin derivatives (lambertianin and di -HHDP-galloyl glucose). These
compoundswere found in both preparations. The most abundant compounds foundwere
ellagitannin derivatives, such as lambertianin and di-HHDP-galloyl glucose. Gasperotti et al.
[3]have identified these compounds in R. fruticosus fruits. To our knowledge, this is the first time
that these compounds were identified and quantified inR. ulmifolius flowers buds.
; Barros, Lillian 
