Optimization of the determination of tocopherols in Agaricus sp. edible mushroo...

• Author(s): Barros, Lillian ; Correia, Daniela M. ; Ferreira, Isabel C.F.R. ; Baptista, Paula ; Santos-Buelga, Celestino
• Date: 2008
• Persistent ID: http://hdl.handle.net/10198/838
• Origin: Biblioteca Digital do IPB
• Subject(s): Vitamin E; HPLC Analysis; Mushrooms
• Document Type: Article
• Language: English

Description

Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.