Document details

Crataegus monogyna buds and fruits phenolic extracts: growth inhibitory activit...

Author(s): Rodrigues, Sandra cv logo 1 ; Calhelha, Ricardo C. cv logo 2 ; Barreira, João C.M. cv logo 3 ; Dueñas, Montserrat cv logo 4 ; Carvalho, Ana Maria cv logo 5 ; Abreu, Rui M.V. cv logo 6 ; Santos-Buelga, Celestino cv logo 7 ; Ferreira, Isabel C.F.R. cv logo 8

Date: 2012

Persistent ID: http://hdl.handle.net/10198/7823

Origin: Biblioteca Digital do IPB

Subject(s): Crataegus monogyna; Phenolic profiles; Human tumor cell lines; Antiproliferative activity


Description
Crataegus monogyna has been extensively studied due to its various alleged health benefits. This study aimed to determine the human tumor cells growth inhibitory activity of phenolic extracts of its flower buds and fruits in three phenological stages, and further characterize the extracts by HPLC–DAD–ESI/MS. Flower bud extract showed the highest antiproliferative activity as indicated by the lowest GI50 values obtained in all the tested cell lines: MCF-7, breast adenocarcinoma; NCI-H460, non-small cell lung cancer; HeLa, cervical carcinoma; HepG2, hepatocellular carcinoma. Furthermore, porcine liver primary cell culture (PLP2) was used to evaluate toxicity to non-tumor cells. Flavonoids, particularly flavonols and flavones (higher in flower buds) and proanthocyanidins (higher in unripe fruits) were the main classes in the studied samples. Phenolic acids (mainly hydroxycinnamic acid derivatives) were also detected in significant amounts, especially in flower bud extract. Regarding anthocyanins, over ripened fruits gave the highest content. The higher bioactivity observed in flower buds might be related with its higher content in phenolic compounds.
Document Type Article
Language English
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