Autor(es):
Rashed, Khaled N. 
; Ćirić, Ana ; Glamočlija, Jasmina ; Calhelha, Ricardo C. ; Ferrreira, Isabel C.F.R. ; Soković, Marina
Data: 2014
Identificador Persistente: http://hdl.handle.net/10198/10155
Origem: Biblioteca Digital do IPB
Assunto(s): Alnus rugosa; Antibacterial activity; Antifungal activity; Anticancer activity; Natural drugs; Phytochemicals
Descrição
This present investigation was undertaken to study antimicrobial and cytotoxic activities ofdichloromethane, ethyl acetate, and methanol 80% extracts from Alnus rugosa L. aerial parts, as wellinvestigate the bio-active compounds of each extract. Antimicrobial activity of the mentioned extractsagainst various bacteria and fungi using microdilution method, and cytotoxicity against five differenthuman tumor cell lines, and in a non-tumor liver cells primary culture were tested. Extracts were sub-jected to phytochemical analysis and column chromatography eluted with different solvents, n-hexane,dichloromethane, ethyl acetate, and methanol gradually. The results showed that the extracts had asignificant antimicrobial activity against all the tested bacteria and fungi, and proved a significant cyto-toxic effect. Methanol 80% extract exhibited the highest antibacterial effect, and ethyl acetate extractwas the most active as antifungal agent while dichloromethane extract showed the lowest antifungaleffect. Dichloromethane extract was the most active against all the tested tumor cell lines. Chromato-graphic separation of dichloromethane extract of A. rugosa aerial parts resulted in the isolation andidentification of -sitosterol, -sitosterol 3-O- -glucoside and apigenin, while from ethyl acetate extract,diosmetin, naringenin, luteolin, and luteolin 7-O- -glucoside were identified, and from methanol 80%extract, quercetin 3-O- -rhamnoside, isorhamnetin 3-O- -glucoside and isorhamnetin 3-O- -glucoside7-O- -rhamnoside were identified. In conclusion, the present research provided significant informationabout antimicrobial and cytotoxic activities and compounds isolated from A. rugosa aerial parts extracts.
