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DNA-based biosensor for the electrocatalytic determination of antioxidant capac...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Lobo-Castañón, M. J.; Miranda-Ordieres, A. J.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the...
Towards a reliable technology for antioxidant capacity and oxidative damage eva...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.To counteract and prevent the deleterious effect of free radicals the living organisms have developed complex endogenous and exogenous antioxidant systems. Several analytical methodologies have been proposed in order to quantify antioxidants in food, beverages and biological fluids. This paper revises the electroanalytical approaches developed for the assessment of the total or individual antioxidant capacity. ...
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