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Compartmentation of glycolysis and glycogenolysis in the perfused rat heart
Anousis, Nick; Carvalho, Rui A.; Zhao, Piyu; Malloy, Craig R.; Sherry, A. DeanDeveloping methods that can detect compartmentation of metabolic pathways in intact tissues may be important for understanding energy demand and supply. In this study, we investigated compartmentation of glycolysis and glycogenolysis in the isolated perfused rat heart using 13C NMR isotopomer analysis. Rat hearts previously depleted of myocardial glycogen were perfused with 5.5 mm [U-13C]glucose plus 50 mU/mL i...
A (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered...
Carvalho, Rui A.; Rodrigues, Tiago B.; Zhao, Piyu; Jeffrey, F. Mark H.; Malloy, Craig R.; Sherry, A. DeanRat hearts were perfused with mixtures of [3-(13)C]pyruvate and [3-(13)C]lactate (to alter cytosolic redox) at low (0.5 mM) or high (2.5 mM) Ca(2+) concentrations to alter contractility. Hearts were frozen at various times after exposure to these substrates, were extracted, and were then analyzed by (13)C NMR spectroscopy. The time-dependent multiplets observed in the (13)C NMR resonances of glutamate in all he...
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