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Immobilization of proteases with a water soluble–insoluble reversible polymer f...
Silva, Carla J. S. M.; Zhang, Qinghua; Shen, J.; Paulo, Artur CavacoA commercial protease, Esperase, was covalently linked to Eudragit S-100, a reversible soluble–insoluble polymer by carbodiimide coupling. When compared to the native enzyme, the immobilized form presented a lower specific activity towards high molecular weight substrates but a higher thermal stability at all temperatures tested. The optimum pH of the immobilized protease was shifted towards the alkaline side b...
Data: 2006
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Origem: RepositóriUM - Universidade do Minho
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