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Structural stability of adenylate kinase from the sulfate-reducing bacteria Des...
Moura, José J. G.; Moura, Isabel; Gavel, Olga Yu.; Bursakov, Sergey A.; Pina, David G.; Zhadan, Galina G.; Shnyrov, Valery L.Biophysical Chemistry 110 (2004) 83–92 ; A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co2+/Zn2+-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential sc...
Thermostability of cardosin A from Cynara cardunculus L.
Pina, David G.; Oliveira, Claudia S.; Sarmento, Ana C.; Barros, Marlene; Pires, Euclides; Zhadan, Galina G.; Villar, Enrique; Gavilanes, FranciscoThe structural stability of cardosin A, a plant aspartic proteinase (AP) from Cynara cardunculus L., has been investigated by high-sensitivity differential scanning calorimetry, intrinsic fluorescence and circular dichroism spectroscopy, and enzymatic activity assays. Even though the thermal denaturation of cardosin A is partially irreversible, valid thermodynamic data can be obtained within a wide pH region. A...
Comparative calorimetric study of non-amyloidogenic and amyloidogenic variants ...
Shnyrov, Valery L.; Villar, Enrique; Zhadan, Galina G.; Sanchez-Ruiz, Jose M.; Quintas, Alexandre; Saraiva, Maria João M.; Brito, Rui M. M.Familial amyloidotic polyneuropathy (FAP) is an autosomal dominant hereditary type of amyloidosis involving amino acid substitutions in transthyretin (TTR). V30M-TTR is the most frequent variant, and L55P-TTR is the variant associated with the most aggressive form of FAP. The thermal stability of the wild-type, V30M-TTR, L55P-TTR and a non-amyloidogenic variant, T119M-TTR, was studied by high-sensitivity differ...
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