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Micropropagation of a recalcitrant pine (Pinus pinea L.): An overview of the ef...

Ragonezi, C.; Caldeira, A.T.; Martins, M.R.; Teixeira, D.; Dias, L.S.; Miralto, O.; Ganhão, E.; Klimaszewska, K.; Zavattieri, A.

Stone pine (Pinus pinea L.) is an economically important forest species in some regions of Iberian Peninsula. Portugal and Spain have nearly 500,000 ha of stone pine stands, representing 85% of worldwide distribution. The main use of this species is for the production of seeds (pinion) for food industry. In addition to its enormous profitability as a producer of seeds, it has beneficial impact on soil protectio...


Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and ...

Ragonezi, C.; Caldeira, A.T.; Martins, M.R.; Dias, L.S.; Santos-Silva, C.; Ganhão, E.; Miralto, O.; Pereira, I.; Louro, R.; Klimaszewska, K.

Stone pine (Pinus pinea L.) is an economically important forest tree in the Mediterranean region and has been the target of breeding and selection through micropropagation mainly for its ecological and ornamental aspects. A crucial step in micropropagation is adventitious rooting of microshoots, which often is highly inefficient in most conifer species including stone pine. Hence, we conducted in vitro co-cultu...


Mycorriha-like structures during in vitro culture of stone pine (Pinus pinea L....

Zavattieri, A.; Miralto, M.O.; Ragonezi, C.; Klimaszewska, K.; Dias, L.S.; Pereira, I.

Dichotomous branching of short lateral roots and the formation of coralloid organs are diagnostic of ectomycorrhizas in many pine species, but the micorrhyzae-like structures found in non mycorrhizated plants of pinus plants show a striking similarity to those of ectomycorrhizas. This phenomenon has been observed previously in other pine species and might be indicative of the long co-evolution of these two king...


Daucus carota L. – An old model for cell reprogramming gains new importance thr...

Costa, J. H.; Cardoso, H. G.; Campos, M.D.; Zavattieri, A.; Frederico, A. M.; Fernandes de Melo, D.; Arnholdt-Schmitt, B.

The paper highlights Daucus carota L. as an ideal model to complement plant stress research on Arabidopsis thaliana L. Recently, alternative oxidase (AOX) is discussed as functional marker candidate for cell reprogramming upon stress. Carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both AOX sub-family genes. We point to recentl...


La embriogénesis somática como via de regeneración clonal de espécies forestales

CELESTINO, C.; HERNÁNDEZ, I.; LÓPEZ-VELA, D.; JIMÉNEZ, J.; ALEGRE, J.; PEIXE, A.; ZAVATTIERI, A.; TORIBIO, M.; CARNEROS, E.

La mejora y conservación de recursos genéticos en especies forestales lleva siglos de retraso con respecto a las especies agrícolas. Los recursos forestales se han considerado tradicionalmente como recursos mineros , en los que primaba la mera extracción dejando exclusivamente a la regeneración natural la labor de sostenibilidad en los montes y dehesas o montados. Hoy en día, el necesario desarrollo del medio ...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia