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Effect of NaCl additive on properties of aqueous PEG–sodium sulfate two-phase s...

Ferreira, Luísa A.; Parpot, Pier; Teixeira, J. A.; Mikheeva, Larissa M.; Zaslavsky, Boris Y.

The concentrations of all components in the phases of aqueous two-phase polyethylene glycol–sodium sulfate system of a fixed composition with different concentrations of NaCl additive were determined. Solvatochromic solvent features of aqueous media in the phases of all the systems were characterized in terms of solvent dipolarity/polarizability, solvent hydrogen bond donor acidity and hydrogen bond acceptor ba...


Effect of salt additives on partition of nonionic solutes in aqueous PEG–sodium...

Ferreira, Luísa A.; Teixeira, J. A.; Mikheeva, Larissa M.; Chait, Arnon; Zaslavsky, Boris Y.

Partition of 12 nonionic organic compounds in aqueous PEG-8000–Na2SO4 two-phase system was examined. Effects of four salt additives (NaCl, NaSCN, NaClO4, and NaH2PO4) in the concentration range from 0.027 up to ca. 1.9 M on binodal curve of PEG-sulfate two-phase system and solute partitioning were explored. It was found that different salt additives at the relatively high concentrations display different effect...


Correlations between distribution coefficients of various biomolecules in diffe...

Madeira, Pedro; Teixeira, J. A.; Macedo, Eugénia; Mikheeva, Larissa M.; Zaslavsky, Boris Y.

Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distri...


ΔG(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systems

Madeira, Pedro; Teixeira, J. A.; Macedo, Eugénia; Mikheeva, Larissa M.; Zaslavsky, Boris Y.

Phase diagrams were determined for aqueous two-phase systems (ATPSs) formed by different paired combinations of Dextran (Dex-75), Ficoll-70, polyethylene glycol (PEG-8000), hydroxypropyl starch (PES-100), and Ucon50HB5100 (a random copolymer of ethylene glycol and propylene glycol) all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 °C. Partition coefficients of a series of dinitrophenylated (D...


“On the collander equation”: protein partitioning in polymer/polymer aqueous tw...

Madeira, Pedro; Teixeira, J. A.; Macedo, Eugénia; Mikheeva, Larissa M.; Zaslavsky, Boris Y.

Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, p...


Relationships between partition coefficients of biomolecules in several aqueous...

Madeira, Pedro; Teixeira, J. A.; Macedo, Eugénia; Mikheeva, Larissa M.; Zaslavsky, Boris Y.


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