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Liquid-liquid equilibrium of aqueous polymer two-phase systems using the modifi...

Madeira, Pedro; Xin Xu; You-Ting Wu; Teixeira, J. A.; Macedo, Eugénia

The modified Wilson equation, developed in a previous publication, for the excess Gibbs energy of aqueous polymer solutions, is extended to multicomponent mixtures. The model provides a flexible framework to correlate and predict phase equilibria of aqueous polymer two-phase systems. The results obtained with the model are in fair agreement with the experimental data.


Liquid-liquid equilibrium phase diagrams of new aqueous two-phase systems : Uco...

Pereira, Martinha; You-Ting Wu; Madeira, Pedro; Venâncio, Armando; Macedo, Eugénia; Teixeira, J. A.

The phase diagrams of Ucon 50-HB-5100 (Ucon) + ammonium sulfate (NH4)2SO4 + water, Ucon 50-HB- 5100 + poly(vinyl alcohol) 10000 (PVA 10000) + water, Ucon 50-HB-5100 + hydroxypropyl starch (Reppal PES 100) + water, and poly(ethylene glycol) 8000 (PEG 8000)-PVA 10000 systems were determined at 22 °C. Measurements at 30 and 40 °C were also done for each Ucon-salt system. A linear regression was used to calculate t...


Aqueous two-phase extraction using thermoseparating polymer : a new system for ...

Pereira, Martinha; You-Ting Wu; Venâncio, Armando; Teixeira, J. A.

Aqueous two-phase partitioning of endo-polygalacturonase (endo-PG) produced by Kluyveromyces marxianus strains was studied, on systems containing the thermoseparating polymer Ucon 50-HB-5100 (a random copolymer of 50% ethylene oxide (EO) and 50% propylene oxide (PO)) as one of the phase-forming compounds. Ucon 50-HB-5100 (Ucon)–polyvinyl alcohol (PVA 10,000), Ucon 50-HB-5100–hydroxypropyl starch (Reppal PES100)...


Separation of endo-polygalacturonase using aqueous two-phase partitioning

You-Ting Wu; Pereira, Martinha; Venâncio, Armando; Teixeira, J. A.

The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)–polyvinyl alcohol (PVA10 000) and PEG–hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000– PVA10 000 system, endo-PG presented in the fermentation broth...


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