Encontrados 8 documentos, a visualizar página 1 de 1
Adhesion of adipose-derived mesenchymal stem cells to patterned protein-glycosa...
Costa, Diana Soares da; Márquez-Posadas, M. C.; Araújo, A. R.; Yang, Y.; Becher, J.; Schnabelrauch, M.; Merino, S.; Groth, T.; Reis, R. L.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; Proteins and glycosaminoglycans (GAGs) are the main constituents of the extracellular matrix (ECM). Until very recently, GAGs were considered as pure structural components while proteins have been associated with crucial cell signalling processes. Nowadays, it is well accepted that these two ECM components act in s...
Sulfation degree of glycosaminoglycans triggers distinct cytoskeleton organisat...
Costa, Diana Soares da; Yang, Y.; Moller, Stephanie; Groth, T.; Reis, R. L.; Pashkuleva, I.Glycosaminoglycans (GAGs) comprise the closest cellular environment: they are building elements of the ECM and can be also found on cells surface. Their biological activity depends on several parameters among which the negative charge is of prime importance[1]. This charge is generally associated with the presence of sulfate groups (-OSO3H). Sulfation is a dynamic modification: it can occur at various positions...
Dynamic culture of osteogenic cells in biomimetically coated poly(caprolactone)...
Araújo, J. V.; Reis, C. Cunha; Rada, Tommaso; Silva, Marta Alves da; Gomes, Manuela E.; Yang, Y.; Ashammakhi, N.; Reis, R. L.; El-Haj, Alicia J.In our previous work, biomimetic calcium phosphate-coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared a...
Genetic variations of EBV-LMP1 from nasopharyngeal carcinoma biopsies: potentia...
Tang,Y.L.; Lu,J.H.; Cao,L.; Wu,M.H.; Peng,S.P.; Zhou,H.D.; Huang,C.; Yang,Y.X.; Zhou,Y.H.; Chen,Q.; Li,X.L.; Zhou,M.; Li,G.Y.To find Epstein-Barr virus (EBV) strains with genetic variations of EBV latent membrane protein 1 (EBV-LMP1) from nasopharyngeal carcinoma (NPC), the full-length DNA of LMP1 genes from 21 NPC biopsies obtained in Hunan province in southern China was amplified and sequenced. Our sequences were compared to those previously reported by the Clustal V method. Results showed that all 21 sequences displayed two amino ...
Plasma surface modification of poly(D,L-lactic acid) as a tool to enhance prote...
Alves, C. M.; Yang, Y.; Marton, D.; Carnes, D. L.; Ong, J. L.; Sylvia, V. L.; Dean, D. D.; Reis, R. L.; Agrawal, C. M.We have studied the influence of oxygen radio frequency glow discharge (RfGD) on the surface and bulk properties of poly(D,L-lactic acid) (PDLLA) and the effect of this surface modification on both protein adsorption and bone cell behavior. PDLLA films were characterized before and after plasma surface modification by water contact angle, surface energy, and adhesion tension of water as well as by scanning elec...
Glass transition of semi-crystalline PLLA with different morphologies as studi...
Picchioci, R.; Yang, Y.; Rodriguez-Perez, M. A.; Alves, N. M.; Mano, J. F.Poly(L-lactic acid) was crystallized from the glassy state at different temperatures to produce fully transformed semi-crystalline specimens exhibiting different lamellar morphologies. The materials were tested by dynamic mechanical analysis, where a Tg decrease was found with an increasing crystallization temperature. Considering a three-phase model, this tendency was related to the corresponding increase in t...
Modulating bone cells response onto starch-based biomaterials by surface plasma...
Alves, C. M.; Yang, Y.; Carnes, D. L.; Ong, J. L.; Sylvia, V. L.; Dean, D. D.; Agrawal, C. M.; Reis, R. L.The effect of oxygen-based radio frequency glow discharge (rfGD) on the surface of different starch-based biomaterials (SBB) and the influence of proteins adsorption on modulating bone–cells behavior was studied. Bovine serum albumin, fibronectin and vitronectin were used in single and complex protein systems. RfGD-treated surfaces showed to increase in hydrophilicity and surface energy when compared to non-mod...
Influence of porosity and fibre diameter on the degradation of chitosan fibre-m...
Reis, C. Cunha; Tuzlakoglu, K.; Baas, E.; Yang, Y.; El Haj, A.; Reis, R. L.The state of the art approaches for tailoring the degradation of chitosan scaffolds are based on altering the chemical structure of the polymer. Nevertheless, such alterations may lead to changes in other properties of scaffolds, such as the ability to promote cell adhesion. The aim of this study was to investigate the influence of physical parameters such as porosity and fibre diameter on the degradation of ch...
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