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Silk Fibroin/Nano-CaP Bilayered scaffolds for osteochondral tissue engineering
Yan, Leping; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.In this study, bilayered silk and silk/nano-CaP scaffolds were developed for osteochondral (OC) tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nano-CaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed tha...
Bioactive macro/micro porous silk fibroin/Nano-sized calcium phosphate scaffold...
Yan, Leping; Correia, Joana Silva; Correia, C.; Caridade, S. G.; Fernandes, E. M.; Sousa, R. A.; Mano, J. F.; Oliveira, Joaquim M.; Oliveira, A. L.Aim: The development of novel silk/nano-sized calcium phosphate (silk/nano-CaP) scaffolds with highly dispersed CaP nanoparticles in the silk fibroin (SF) matrix for bone tissue engineering. Materials & methods: Nano-CaP was incorporated in a concentrated aqueous SF solution (16 wt.%) by using an in situ synthesis method. The silk/nano-CaP scaffolds were then prepared through a combination of salt-leaching/ lyo...
De novo bone formation on macro/microporous silk and silk/nano-sized calcium ph...
Yan, Leping; Salgado, A. J.; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.Macro/micro porous silk/nano-sized calcium phosphate scaffolds (SC16) with bioactive and superior physicochemical properties have been recently developed. In this study, we aim at evaluating the new bone formation ability of the SC 16 scaffolds in vivo, using silk fibroin scaffolds (S16) as control. The CaP distribution profile in the scaffolds was characterized by Micro-Computed Tomography. The in vitro minera...
Silk-Fibroin/Methacrylated Gellan Gum Hydrogel as an novel scaffold for applica...
Pereira, H.; Correia, Joana Silva; Yan, Leping; Caridade, S. G.; Frias, A. M.; Oliveira, A. L.; Mano, J. F.; Oliveira, Joaquim M.Introduction: Knee meniscus injury is highly prevalent and there is a demand for new cost-effective treatment solutions. Tissue engineering (TE) and regenerative medicine strategies using acellular scaffolds are being used in clinical application for total or partial meniscus replacement [1]. Although this strategy has been considered as a safe and promising approach, progressive volume reduction of the implant...
Macro/microporous silk fibroin scaffolds with potential for articular cartilage...
Yan, Leping; Oliveira, Joaquim M.; Oliveira, Ana Leite; Caridade, S. G.; Mano, J. F.; Reis, R. L.This study describes the developmental physicochemical properties of silk fibroin scaffolds derived from high concentration aqueous silk fibroin solutions. The silk fibroin scaffolds were prepared with different initial concentrations (8%, 10%, 12% and 16% (wt%)) and obtained by combining the salt-leaching and freeze-drying methodologies. The results indicated that the antiparallel β-pleated sheet (silk-II) con...
Development of macro/micro porous silk fibroin scaffolds with nano-sized calciu...
Yan, Leping; Correia, Cristina; Correia, Joana Silva; Caridade, S. G.; Fernandes, E. M.; Mano, J. F.; Sousa, R. A.; Oliveira, Joaquim M.Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combination of sa...
Advanced mimetic materials for meniscus tissue engineering : targeting segmenta...
Correia, Joana Silva; Pereira, H.; Yan, Leping; Gonçalves, Vera M.; Oliveira, A. L.; Oliveira, Joaquim M.; Reis, R. M.; Mendes, João EspregueiraMeniscus lesions are among the most common orthopaedic injuries which can ultimately lead to degeneration of the knee articular cartilage. The human meniscus has a limited healing potential, partly due to a poor vasculature, and thus meniscus regeneration using tissue engineering strategies has recently been investigated as a promising alternative to total/partial meniscectomy [1]. Advanced scaffolds for tissue...
Development of a bilayered scaffold based on silk fibroin and silk fibroin/nano...
Yan, Leping; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.Objectives: Osteochondral defect is a common condtion in clinic. Satisfactory outcomes are rarely achieved by traditional methods. Tissue engineering might be a promising strategy for this hinder. The aim of this study is to mimick the stratified structure of osteochondral tissue, by developing a bilayered scaffold for osteochondral regeneration. The developed bilayered scaffold is composed of a porous silk fib...
Development of silk-based scaffolds for tissue engineering of bone from human a...
Correia, Cristina; Bhumiratana, Sarindr; Yan, Leping; Oliveira, A. L.; Gimble, Jeffrey M.; Rockwood, Danielle; Kaplan, David; Sousa, R. A.; Reis, R. L.Silk fibroin is a potent alternative to other biodegradable biopolymers for bone tissue engineering (TE), because of its tunable architecture and mechanical properties, and its demonstrated ability to support bone formation both in vitro and in vivo. In this study, we investigated a range of silk scaffolds for bone TE using human adipose-derived stem cells (hASCs), an attractive cell source for engineering auto...
Macro/micro porous silk fibroin scaffolds obtained via combined methodologies f...
Yan, Leping; Oliveira, Joaquim M.; Oliveira, Ana Leite; Caridade, S. G.; Mano, J. F.; Reis, R. L.This study proved that the salt leached silk fibroin scaffolds can be developed by using more than 10 wt% aqueous silk fibroin solutions. There were some previous studies shown that this was impossible. ; This work has shown that the developed silk fibroin scaffolds can combine adequate mechanical properties with a unique morphology which make them suitable to be used in tissue engineering applications, partic...
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