Encontrados 9 documentos, a visualizar página 1 de 1
Cell-free layer (CFL) measurements in complex geometries: contractions and bifu...
Novais, Susana; Pinho, Diana; Bento, David; Pinto, Elmano; Yaginuma, Tomoko; Fernandes, Carla S.; Garcia, Valdemar; Pereira, Ana I.; Lima, JoséIn this chapter we discuss the cell-free layer (CFL) developed adjacent to the wall of microgeometries containing complex features representative of the microcirculation, such as contractions, expansions, bifurcations and confluences. The microchannels with the different geometries were made of polydimethylsiloxane (PDMS) and we use optical techniques to evaluate the cell-free layer for red blood cells (RBC) su...
Cell-free layer measurements in a bifurcation microchannel : comparison between...
Bento, David; Pinho, Diana; Pinto, Elmano; Yaginuma, Tomoko; Correia, Teresa Montenegro; Lima, José; Pereira, Ana I.; Lima, R.In the present work, in vitro blood flowing through a bifurcation microchannel was studied. The aim was to measure the Trajectories of the cell-free layer (CFL) by using different methods, i. e., a manual and two automatic methods.
Cell-free layer measurements in a bifurcation microchannel : comparison between...
Bento, David; Pinho, Diana; Pinto, Elmano; Yaginuma, Tomoko; Correia, Teresa Montenegro; Lima, José; Pereira, Ana I.; Fernandes, Carla S.O artigo deve também estar associado ao Departamento de Electrotécnia, Tecnologia Mecânica e Química ; In the present work, in vitro blood flowing through a bifurcation microchannel was studied. The aim was to measure the Trajectories of the cell-free layer (CFL) by using different methods, i. e., a manual and two automatic methods.
Measurement of red blood cells deformation index in a hyperbolic microchannel
Faustino, V.; Pinho, Diana; Yaginuma, Tomoko; Calhelha, Ricardo C.; Man-Kim, Gyeong; Arana, Sergio; Lima, R.; Ferreira, Isabel C.F.R.; Oliveira, M.S.N.A hyperbolic microchannel was used for the purpose of observing the deformation index of the erythrocytes after contact with human tumour cell line (HCT-15 - colon carcinoma). Three cases were compared and the results showed that the deformation index decreases when erythrocytes are in contact with HCT-15.
Separation and deformation of red blood cells in PDMS microchannels
Rodrigues, Raquel Oliveira; Faustino, V.; Pinho, Diana; Pinto, Elmano; Cidre, Diana; Yaginuma, Tomoko; Taboada, B.; Bento, David; Lima, R.Over the years, several experimental techniques were performed in in vitro environments, in an attempt to understand the flow behaviour of blood in microcirculation. Several of these studies were performed in glass capillaries, and have produced significant results with respect to rheological properties of blood [1, 2]. Another way to perform in vitro blood studies is to use microchannels fabricated by soft- li...
Human red blood cell behavior under homogeneous extensional flow in a hyperboli...
Yaginuma, Tomoko; Oliveira, M. S. N.; Lima, R.; Ishikawa, T.; Yamaguchi, T.It is well known that certain pathological conditions result in a decrease of red blood cells (RBCs) deformability and subsequently can significantly alter the blood flow in microcirculation, which may block capillaries and cause ischemia in the tissues. Microfluidic systems able to obtain reliable quantitative measurements of RBC deformability hold the key to understand and diagnose RBC related diseases. In th...
A microfluidic device for partial cell separation and deformability assessment
Pinho, D.; Yaginuma, Tomoko; Lima, R.Blood flow in microcirculation shows several interesting phenomena that can be used to develop microfluidic devices for blood separation and analysis in continuous flow. In this study we present a novel continuous microfluidic device for partial extraction of red blood cells (RBCs) and subsequent measurement of RBC deformability. For this purpose, we use polydimethylsiloxane (PDMS) microchannels having differen...
Visualization of the cell-free layer (CFL) in a PDMS microchannel with a micro-...
Pinho, Diana; Yaginuma, Tomoko; Faustino, V.; Pinto, Elmano; Rodrigues, Raquel Oliveira; Cidre, Diana; Pereira, Ana I.; Lima, R.Red blood cells (RBCs) have a tendency to undergo axial migration due to the parabolic velocity profile which results in a high shear stress around wall that forces the RBC to move towards the center induced by the tank treading motion of the RBC membrane. As a result there is a formation of cell-free layer (CFL) with extremely low concentration of cells. Based on this phenomenon several works have proposed mic...
Flow of red blood cells through a microfluidic extensional device: an image ana...
Yaginuma, Tomoko; Pereira, Ana I.; Rodrigues, P.J.; Lima, R.; Oliveira, M.S.N.; Ishikawa, Takuji; Yamaguchi, TakamiThe present study aims to assess the deformability of red blood cells (RBCs) under extensionally dominated microfluidic flows using an image based technique. For this purpose, a microchannel having a hyper-bolic shaped-contraction was used and the images were captured by a standard high-speed microscopy system. The images acquired display RBCs with various light intensity levels and image analysis was used to q...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||