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Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514 : kinetics an...
Rodrigues, A. C.; Wuertz, Stefan; Brito, A. G.; Melo, L. F.Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 ± 0.1 mg Lˉ¹ hˉ¹, higher than the apparent degradation rate of fluorene, 0.8 ± 0.07 mg Lˉ¹ hˉ¹. The ro...
Three-dimensional distribution of GFP-labeled Pseudomonas putida during biofilm...
Rodrigues, A. C.; Wuertz, Stefan; Brito, A. G.; Melo, L. F.Confocal laser scanning microscopy was used to monitor the colonization pattern of the gfplabeled derivative strain of Pseudomonas putida ATCC 17514 on fluorene and phenanthrene crystals. The in situ experiments showed that P. putida tends to grow directly on phenanthrene, forming a biofilm on accessible crystalline surfaces. On the other hand, no significant biofilm formation was observed in the presence of fl...
Three-dimensional distribution of GFP-tagged Pseudomonas putida during biofilm ...
Rodrigues, A. C.; Wuertz, Stefan; Brito, A. G.; Melo, L. F.Confocal laser scanning microscopy was used to monitor the colonization pattern of the gfp-labelled derivative strain of Pseudomonas putida ATCC 17514 on fluorene and phenanthrene crystals. The in situ experiments showed that P. putida tends to grow directly on phenanthrene, forming a biofilm on accessible crystalline surfaces. On the other hand, no significant biofilm formation was observed in the presence of ...
Effect of flow regime on the architecture of a pseudomonas fluorescens biofilm
Pereira, M. O.; Kuehn, Martin; Wuertz, Stefan; Neu, Thomas; Melo, L. F.A comparison of the effects of laminar versus turbulent flow regime on the characteristics of a single-species biofilm is presented. The study was carried out by growing Pseudomonas fluorescens biofilms in a flow cell and studying the different layers of the biological matrix with a confocal laser-scanning microscope. The following conclusions were obtained: i) a higher concentration of cells was found in the u...
Nitrifying and heterotrophic population dynamics in biofilm reactors : effects ...
Nogueira, R.; Melo, L. F.; Purkhold, Ulrike; Wuertz, Stefan; Wagner, MichaelTwo biofilmreactors operated with hydraulic retention times of 0.8 and 5.0 h were used to study the links between population dynamics and reactor operation performance during a shift in process operation from pure nitrification to combined nitrification and organic carbon removal. The ammonium and the organic carbon loads were identical for both reactors. The composition and dynamics of the microbial consortia ...
Microbial population dynamics versus nitrification performance in biofilm react...
Nogueira, R.; Purkhold, Ulrike; Wuertz, Stefan; Melo, L. F.; Wagner, Michaelln order to improve biological reactors operation and design it is important to study the effects of changes in process parameters with regard to the microbial community structure and, vice versa, the effects of community structure and dynamics on the reactors performance. Two biofilm reactors operated with hydraulic retention times of 1 h and 6 h were used to study the links between population dynamics and rea...
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