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Characterisation of new aqueous two-phase systems using thermoseparating polymers
Pereira, Martinha; Madeira, Pedro; Wu, You-Ting; Teixeira, J. A.; Macedo, EugéniaAqueous two-phase systems (ATPS) are increasingly being used for separation of proteins, cells and viruses and some small molecular weight substances since they offer a gentle and friendly environment. The use of new thermoseparating polymers opens new opportunities to develop more efficient aqueous two-phase systems as they make possible polymer recycling just by temperature control. Phase diagrams for Ucon-(N...
Production and separation of an endo-polygalacturonase from Kluyveromyces marxi...
Pereira, M. A.; Wu, You-Ting; Venâncio, Armando; Teixeira, J. A.
Recovery of endo-polygalacturonase using polyethylene glycol-salt aqueous two-p...
Wu, You-Ting; Pereira, Martinha; Venâncio, Armando; Teixeira, J. A.The partitioning behaviour of endo-polygalacturonase (endo-PG) and total protein from a clarified Kluyveromyces marxianus fermentation broth in polyethylene glycol (PEG)-ammonium sulfate and PEG-potassium phosphate (pH=7) aqueous two-phase systems was experimentally investigated. Both the enzyme and total protein partitioned in the bottom phase for these two kinds of systems. The enzyme partitioning coefficient...
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