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Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombin...
Cao, Xue-jun; Wu, Xing-yan; Fonseca, Luís Pina; Cabral, J. M. S.; Marcos, João CarlosBioconversion of Penicillin G in PEG 20000-Dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7%(w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U/ml, pH 7.8, temperature 37°C, reaction time 40 min. Five repeated batches could be performed in ...
Data: 2004
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Origem: RepositóriUM - Universidade do Minho
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