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Effect of pulsed electric field on the germination of barley seeds
Dymek, K.; Dejmek, Petr; Panarese, V.; Vicente, A. A.; Wadsö, Lars; Finnie, C.; Gómez Galindo, FedericoThis study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergen...
Exploring metabolic responses of potato tissue induced by electric pulses
Gómez Galindo, Federico; Wadsö, Lars; Vicente, A. A.; Dejmek, PetrIn this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. ...
How Small Polar Molecules Protect Membrane Systems against Osmotic Stress: The ...
Costa-Balogh, Fátima O.; Wennerström, Hakan; Wadsö, Lars; Sparr, EmmaWe investigate how a small polar molecule, urea, can act to protect a phospholipid bilayer system against osmotic stress. Osmotic stress can be caused by a dry environment, by freezing, or by exposure to aqueous systems with high osmotic pressure due to solutes like in saline water. A large number of organisms regularly experience osmotic stress, and it is a common response to produce small polar molecules intr...
The Hydration of a DNA−Amphiphile Complex
Leal, Cecilia; Wadsö, Lars; Olofsson, Gerd; Miguel, Maria; Wennerström, HåkanWe present measurements of isothermal DNA−hexadecyltrimethylammonium (DNACTA) complex and pure DNA hydration at 25 °C using a sorption microcalorimeter. This calorimeter provides simultaneous measurement of (i) water activity (sorption isotherms) and (ii) the partial molar enthalpy of water as a function of water uptake. For pure DNA, hydration is exothermic over the studied concentration range and we find an a...
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