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Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Prot...
Paiva, Gabriel; Proença, Diogo Neves; Francisco, Romeu; Veríssimo, P.; Santos, Susana S.; Fonseca, Luís; Abrantes, I. M. O.; Morais, P. V.Bacteria associated with the nematode Bursaphelenchus xylophilus, a pathogen of trees and the causal agent of pine wilt disease (PWD) may play a role in the disease. In order to evaluate their role (positive or negative to the tree), strains isolated from the track of nematodes from infected Pinus pinaster trees were screened, in vitro, for their nematicidal potential. The bacterial products, from strains more ...
Identification of the cell wall receptor for Candida nodaensis Killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, CândidaComunicação efectuada no XIV Congresso Nacional de Bioquímica em Vilamoura (Portugal), 2004. ; The biological action of the K toxins involves a first step in the killing process, which correspond to the adsorption the toxin to the cell wall of sensitive cells. Here we describe the work performed towards the identification of the cell wall receptor for the zymocin under this study. For this purpose, the main ce...
Partial purification and characterisation of Candida nodaensis Killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, C.Comunicação efectuada no Yeast Genetics and Molecular Biology Meeting, Seattle, 2004. ; Preliminary experiments showed that C. nodaensis K factor keeps its biological activity at high NaCl concentrations and is stable after incubation in a relatively broad range of temperature and pH values, whether or not in the presence of salt. The isolation of this K toxin was already achieved by a experimental protocol i...
Partial purification and characterization of Candida nodaensis killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, C.Comunicação efectuada no Congresso Nacional de Microbiologia em Tomar (Portugal), 2003. ; Preliminary experiments performed to characterize C. nodaensis K factor showed that, besides keeping its biological activity at high NaCl concentrations, is stable after incubation in a relatively broad range of temperature and pH values, whether or not in the presence of salt. In order to achieve the isolation, purificat...
The killer toxin of the halotolerant yeast Candida nodaensis
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, CândidaApresentação efectuada no "XIX International Congress on Yeast Genetics and Molecular Biology", em Gotterborg, Suécia, em 2003. ; Several halotolerant yeast strains, displaying a broader spectrum of K activity in the presence of salt, were identified in a previous survey. The possibility that these zymocins could be more stable than the ones described so far, lead to the selection of the extremely halotolerant...
The killer toxin of the halotolerant yeast Candida nodaensis
Aguiar, Cristina; Silva, Sónia Carina; Veríssimo, P.; Pires, E.; Lucas, CândidaComunicação efectuada no XIII Congresso Nacional de Bioquímica em Lisboa (Portugal), 2002. ; In a previous survey, the halotolerant yeast Candida nodaensis was identified as one of the strongest killer strains, displaying an enhancement of killer action in the presence of salt (maximum activity detected at 2M NaCl). Results of nucleic acid extractions and curing experiments suggested that this zymocin might be...
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