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EFICÁCIA DE COLOIDES VS CRISTALOIDES NA REPOSIÇÃO VOLÉMICA A NÍVEL HEPÁTICO EM ...
Pina R, Vala H; Cruz R, Venâncio C; Silva A, Garcia C; Ortiz A, Ferreira DApesar dos avanços na sua abordagem terapêutica, a hemorragia severa continua a ser a principal causa de morbilidade e mortalidade em animais vítimas de trauma ou sujeitos a intervenção cirúrgica. A causa das lesões decorrentes, ou da morte consequente, deve-se ao deficit de volume de fluidos intravasculares e subsequente desenvolvimento do estado hipovolémico.O controlo da hipovolémia passa pela resolução da h...
Apoptosis as a prognostic marker in prediction of renal injury, after acute ble...
Cruz, R.; Vala, H.; Machado, A.; Venâncio, C.; Mesquita, J.; Silva, A.; Ortiz, A. L.; Ferreira, D.Objectives: The aim of this study is to identify and quantify apoptosis in renal tissue, using a biochemical marker (TUNEL) in a pig haemorrhagic model, after intravascular volume replacement with Ringer's lactate RL) or Hydroxyethylstarch (HES) 130/0.4) solutions. Methods: 18 Large White pigs underwent total intravenous anaesthesia (TIVA) with propofol and remifentanil. 25 ml/kg of arterial blood were removed ...
Small intestine apoptosis evaluation after haemorrhage followed by volume repla...
Ortiz, A.; Vala, Helena; Garcia, C.; Cruz, R.; Venâncio, C.; Silva, A.; Ferreira, D.Apoptosis plays a key role in intestinal injury, as response to ischemia. Furthermore, reperfusion exacerbates abnormal intestinal apoptosis. In this study, the apoptotic index (AI) in the small intestine after haemorrhage and volume replacement with Ringer´s solution was evaluated, in a pig model
Histological evaluation of the spleen after acute bleeding followed by volume r...
Cabral, M.; Ortiz, A.; Venâncio, C.; Mesquita, J.; Nóbrega, C.; Silva, A.; Vala, Helena; Ferreira, D.Spleen is important for many hematopoietic and immunological functions, namely in hemodynamic compensation during hemorrhagic shock, with a crucial role in restoring blood volume in situations of acute haemorrhage. Also, the administration of physiological solutions is of great importance for the correction of circulating volume, avoiding complications due to hypovolemia. The aim of this study was to eval...
Evaluation of small intestine apoptosis after haemorrhage followed by volume re...
Ortiz, A.; Vala, H.; Garcia, C.; Cruz, R.; Venâncio, C.; Silva, A.; Ferreira, D.FEDER funds trough the COMPETE Program, and by national funds from Portuguese Foundation for Science and Technology, under the project COMPETE: FCOMP-01-0124-FEDER-009525 ; Apoptosis plays a key role in intestinal injury, as response to ischemia. Furthermore, reperfusion exacerbates abnormal intestinal apoptosis. In this study, the apoptotic index (AI) in the small intestine after haemorrhage and volume replac...
Renal apoptosis signalling in a pig haemorrhagic model, after volume replacemen...
Cruz, R.; Vala, Helena; Machado, A.; Venâncio, C.; Mesquita, Jr; Silva, A.; Ortiz, A.; Ferreira, D.TUNEL staining is one of the most frequently used method to detect apoptosis in renal tissues. The aim of this part of the study was to evaluate kidney damage after acute bleeding and volume replacement, with a colloid or a crystalloid, in a pig model
Evaluation and characterization of intestinal oedema in pigs
Ortiz, A. L.; Vala, Helena; Silva, A.; Venâncio, C.; Garcia, C.; V, D.Introduction When characterizing small intestine early lesions in studies concerning intestinal hypoperfusion in pigs, authors faced a significant difficulty when trying to standard the oedema grading to allow an accurate comparison between groups. Therefore, specific alterations were assumed to characterize grade1 (G1), grade2 (G2) and grade3 (G3) oedema. Material and Methods Eighteen Large White pigs underwen...
Volume replacement with hydroxyethyl starch (HES) 130/0.4 and ringer lactate so...
Oliveira, A.; Venâncio, C.; Silva, A.; Souza, A.; Vala, Helena; Ferreira, D.Background and goal of the study: Perioperative and intraoperative hypovolemia may cause intestinal hypoperfusion and postoperative complications1. The objective of this study is to analyse the effect on the small bowel mucosa of HES 130/0.4 and Ringer solutions used for volume replacement after severe haemorrhage in pigs. Materials and methods: Six Large White pigs under TIVA w ith propofol 15 mg/kg/h and remi...
Estimation of rabbit hind leg muscle weight and muscularity by real time ultras...
Silva, S.R; Guedes, C.M; Mourão, J.L; Venâncio, C; Pinheiro, V.MForty-four New Zealand White x Californian rabbits were used to evaluate the real time ultrasonography (RTU) in assessing hind leg muscle weight and hind leg muscularity. For in vivo muscularity determination animals were scanned over the hind leg using a RTU machine equipped with a 5 MHz probe. The RTU images were taken on hind leg at three positions perpendicular to femur bone. At each position a section of h...
Application of the wavelet image analysis technique to monitor cell concentrati...
Garófano,G. J. R.; Venancio,C. G.; Suazo,C. A. T.; Almeida,P. I. F.The growth of cells of great practical interest, such as, the filamentous cells of bacterium Streptomyces clavuligerus, the yeast Saccharomyces cerevisiae and the insect Spodoptera frugiperda (Sf9) cell, cultivated in shaking flasks with complex media at appropriate temperatures and pHs, was quantified by the new wavelet transform technique. This image analysis tool was implemented using Matlab 5.2 software to ...
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