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Platelet iysate maintains chondrogenic potential and promotes cartilage regener...

Pereira, R. C.; Scaranari, M.; Strada, P.; Reis, R. L.; Cancedda, Ranieri; Gentili, Chiara

cartilage. We report the biological effect of the platelet lysate (PL), a PRP derivative, on primary human articular chondrocytes (HAC) cultured under both physiological and inflammatory condition. Added to the culture medium, PL induced a strong mitogenic response in the chondrocytes. The in vitro expanded cell population maintained a chondrogenic re-­‐differentiation potential as revealed by micromass culture...


Encapsulation of human articular chondrocytes into 3D hydrogel : phenotype and...

Pereira, Rui C.; Gentili, Chiara; Cancedda, Ranieri; Azevedo, Helena S.; Reis, R. L.

This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regenera...


Novel injectable gel (system) as a vehicle for human articular chondrocytes in ...

Pereira, Rui C.; Scaranari, M.; Castagnola, P.; Grandizio, M.; Azevedo, Helena S.; Reis, R. L.; Cancedda, Ranieri; Gentili, Chiara

We developed a novel injectable carrageenan/fibrin/hyaluronic acid-based hydrogel with in situ gelling properties to be seeded with chondrogenic cells and used for cartilage tissue engineering applications. We first analysed the distribution within the hydrogel construct and the phenotype of human articular chondrocytes (HACs) cultured for 3 weeks in vitro. We observed a statistically significant increase in th...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia