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Diversity and antibiotic resistance of Acinetobacter spp. in water from the sou...

Da Rocha, C. A. Narciso; Vaz-Moreira, Ivone; Svensson-Stadler, L.; Moore, E. R. B.; Manaia, C. M.


Diversity and antibiotic resistance of aeromonas spp. in drinking and waste wat...

Figueira, Vânia; Vaz-Moreira, Ivone; Silva, Márcia; Manaia, Célia M.

The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene sequences. Resis...


Diversity and antibiotic resistance patterns of sphingomonadaceae isolates from...

Vaz-Moreira, Ivone; Nunes, Olga C.; Manaia, Célia M.

Sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the g...


Culture-dependent and culture-independent diversity surveys target different ba...

Vaz-Moreira, Ivone; Egas, Conceição; Nunes, Olga C.; Manaia, Célia M.

Compared with culture-independent approaches, traditionally used culture-dependent methods have a limited capacity to characterizewatermicrobiota. Nevertheless, for almost a century the latter have been optimized to detect and quantify relevant bacteria. A pertinent question is if culture-independent diversity surveys give merely an extended perspective of the bacterial diversity or if, even with a higher cover...


Microbacterium invictum sp. nov., isolated from homemade compost

Vaz-Moreira, Ivone; Lopes, Ana R.; Faria, Cátia; Sproer, Cathrin; Schumann, Peter; Nunes, Olga C; Manaia, Célia M.

Strain DC-200T was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. The isolate was a Gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10–37 6C, pH 6.0–9.5 and with up to 5% of NaCl. The peptidoglycan was of the type B1 alpha and the muramic acid residues were glycolylated. The major f...


Antibiotic resistance in coagulase negative staphylococci isolated from wastewa...

Faria, Cátia; Vaz-Moreira, Ivone; Serapicos, Eduarda; Manaia, Célia M.

This study reports the antibiotic resistance patterns of coagulase negative staphylococci (CNS) isolated from a drinking water treatment plant (WTP), a drinking water distribution network, responsible for supplying water to the consumers (WDN), and a wastewater treatment plant (WWTP), responsible for receiving and treating domestic residual effluents. Genotyping and the 16S rRNA gene sequence analysis demonstra...


Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost

Vaz-Moreira, Ivone; Nobre, M. Fernanda; Ferreira, António C. Silva

A bacterial strain isolated from sewage sludge compost, strain SC-083T, was characterized. The isolate was a motile, Gram-positive, short rod, forming coryneform V-shaped cells during the early stages of growth. The organism was strictly aerobic and able to grow between 22 and 36 °C and between pH 5.5 and 8.0. The predominant fatty acids were cyclohexyl-C17 : 0, anteiso-C17 : 0 and iso-C16 : 0, the major respir...


Microbacterium luticocti sp. nov., isolated from sewage sludge compost

Vaz-Moreira, Ivone; Lopes, Ana R.; Falsen, Enevold; Schumann, Peter; Nunes, Olga C.; Manaia, Célia M.

Strain SC-087BT, isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. The isolate was a Gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27–45 6C, pH 5.5–9.7 and in up to 10% NaCl. The peptidoglycan was of the B2b type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. The mur...


Diversity of bacterial isolates from commercial and homemade composts

Vaz-Moreira, Ivone; Silva, Maria E.; Manaia, Célia M.; Nunes, Olga C.

The diversity of heterotrophic bacterial isolates of three commercial and two homemade composts was studied. The commercial composts were produced from poultry litter (PC), sewage sludge (SC), municipal solid waste (MC), and homemade composts (thermal compost [DC] and vermicompost [VC]) from food wastes. The taxonomic and physiological diversity of the heterotrophic culturable bacteria was assessed using phenot...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia