Encontrados 13 documentos, a visualizar página 1 de 2
Cloning, characterization and in vitro and in planta expression of a glucanase ...
Martins, Ivone; Martins, Fátima; Belo, Hélio; Vaz, Madalena; Carvalho, Marisa; Cravador, Alfredo; Choupina, AltinoOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamom...
Lipases: biocatalizadores da hidrólise de triacilglicerois
Vaz, Madalena; Choupina, AltinoOs enzimas lipases (E.C.3.1.1.3) pertencem à família das serinas hidrolases, que por sua vez incluem várias enzimas esterases (E.C.3.1.1.1). Uma característica que distingue as lipases das esterases é o facto de serem capazes de realizar a hidrólise de óleos e gorduras em glicerol e ácidos gordos livres na interface óleo-água. Normalmente é aceite que as esterases hidrolisam ligações éster em esteres e triacilg...
Isolation and analysis of lip2 gene from Trichoderma harzianum
Vaz, Madalena; Belo, Hélio; Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoPTDC/AGR-AAM/67628/2006 - dentification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi
Isolation and analysis of lip2 gene from Trichoderma harzianum
Vaz, Madalena; Belo, Hélio; Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoPTDC/AGR-AAM/67628/2006 - Identification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi ; Trichodorma spp. covers a group of fungi extremely common in soils of all climatic areas. These fungi arc efficient enzyme producers, with industrial applications, or in nature, involved in the degradation of the coli wall of the ph...
A bioinformática na identificação de genes
Vaz, Madalena; Jorge, LurdesCom o objectivo de identificar genes, avaliaram-se nove EST (“Expressed Sequence Tag”) de uma biblioteca de cDNA de Trichoderma harzianum. Numa primeira etapa, as ESTs foram sequenciadas, sendo as sequências analisadas com programas bioinformáticos de análise de similaridade em bancos de dados, utilizando ferramentas como Blast P e Fasta X. Identificaram-se duas ESTs que se enquadravam nos objectivos, sendo uma...
Caracterização de genes através de recursos bioinformáticos
Jorge, Lurdes; Vaz, MadalenaApós descodificação da fase de leitura aberta de um gene, uma série de ferramentas bioinformáticas podem ser utilizadas para a caracterização da sequência deduzida da proteína. Uma pesquisa no website do Expasy Proteomics Server (http://expasy.org/tools) e uma sequência nucleotídica permitem-nos identificar e caracterizar proteínas, identificar motivos, padrões e perfis, inferir a sua estabilidade, localização ...
Characterization of molecular factors from plants pathogen Phytophthora cinnamo...
Choupina, Altino; Martins, Fátima; Vaz, Madalena; Dominguez, Ángel; Martins, IvoneThe culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...
Isolation and characterization of necrosis-inducing phytophthora protein 1 (npp...
Martins, Ivone; Meirinho, Sofia G.; Belo, Hélio; Vaz, Madalena; Martins, Fátima; Choupina, AltinoOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to their particular physiological characteristics, no efficient treatments against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytoph...
Characterization of molecular factors from plants pathogen Phytophthora cinnamomi
Choupina, Altino; Martins, Fátima; Vaz, Madalena; Dominguez, Angel; Martins, I.M.The culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...
Characterization of a glucanase inhibitor protein from Phytophthora cinnamomi i...
Martins, I.M.; Martins, Fátima; Belo, Hélio; Vaz, Madalena; Choupina, AltinoOomycetes from the genus Phytophthora are fungos-likeplant pathogens that are devastating fr agriculture and natural ecosystems. Due to their partucular physiological characteristics, no eficiente tratmentts, it against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phy...
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