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Evaluation of two methods of in vitro production of ovine embryos using fresh o...
Romão, Ricardo; Marques, Carla; Batista, Maria Conceição; Vasques, Maria Irene; Barbas, J Pedro; Horta, António M; Carolino, NunoSuccessful production of high quality blastocysts depends on the use of a culture system that ensures the acquisition of developmental competence by the maturing oocyte followed by an efficient in vitro fertilization. In the present work the effect of FSH and pyruvate in an EGF containing medium for ovine oocyte maturation prior to insemination with fresh(F)or frozen–thawed (FT) semen on embryo developmental co...
Effect of arachidonic acid supplementation and cyclooxygenase/lipoxygenase inhi...
Pereira,Rosa Maria; Marques,Carla Cruz; Baptista,Maria da Conceição; Vasques,Maria Irene; Horta,António EduardoThe effect of arachidonic acid (AA) cascade on bovine embryo development in a granulosa cell co-culture system was studied. Arachidonic acid (100 µM) was supplemented from 1-cell to 8-16 cell block stage (first three days of co-culture) and from 1-cell to hatching. Specific cyclooxygenase (indomethacin, 28 µM) and lipoxygenase (nordihydroguaiaretic acid - NDGA, 28 µM) inhibitors were used from 1-cell to 8-16 ce...
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