Encontrados 12 documentos, a visualizar página 1 de 2
The use of Keratin in biomedical applications
Vasconcelos, Andreia; Paulo, Artur CavacoKeratins are naturally derived proteins that can be fabricated into several biomaterials morphologies including films, sponges and hydrogels. As a physical matrix, keratin biomaterials have several advantages of both natural and synthetic materials that are useful in tissue engineering and controlled released applications. Like other naturally derived protein biomaterials, such as collagen, keratin possess amin...
Novel silk fibroin/elastin wound dressings
Vasconcelos, Andreia; Gomes, Andreia; Paulo, Artur CavacoSilk fibroin (SF) and elastin (EL) scaffolds were successfully produced for the first time for the treatment of burn wounds. The self-assembly properties of SF, together with the excellent chemical and mechanical stability and biocompatibility, were combined with elastin protein to produce scaffolds with the ability to mimic the extracellular matrix (ECM). Porous scaffolds were obtained by lyophilization and we...
Protein matrices for wound dressings
Vasconcelos, AndreiaTese de doutoramento em Engenharia Têxtil ; Fibrous proteins such as silk fibroin (SF), keratin (K) and elastin (EL) are able to mimic the extracellular matrix (ECM) that allows their recognition under physiological conditions. The impressive mechanical properties, the environmental stability, in combination with their biocompatibility and control of morphology, provide an important basis to use these proteins...
Wound dressings for a proteolytic-rich environment
Vasconcelos, Andreia; Paulo, Artur CavacoWound dressings have experienced continuous and significant changes over the years based on the knowledge of the biochemical events associated with chronic wounds. The development goes from natural materials used to just cover and conceal the wound to interactive materials that can facilitate the healing process, addressing specific issues in non-healing wounds. These new types of dressings often relate with th...
Protein disulphide isomerase-assisted functionalization of keratin-based matrices
Fernandes, Margarida M.; Gomes, A. C.; Vasconcelos, Andreia; Munteanu, Florentina-Daniela; Tzanov, Tzanko; Gonçalves, M. Sameiro T.; End, N.In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (isomerase activity), leading proteins towards their native configuration. In this study, PDI was used to attach cysteine-containing compounds (CCCs) onto hair, to enhance compound migration within hair fib...
Tailoring elastase inhibition with synthetic peptides
Vasconcelos, Andreia; Azoia, Nuno G.; Carvalho, A. C.; Gomes, A. C.; Guebitz, G. M.; Paulo, Artur CavacoChronic wounds are the result of excessive amounts of tissue destructive proteases such as human neutrophil elastase (HNE). The high levels of this enzyme found on those types of wounds inactivate the endogenous inhibitor barrier thus, the search for new HNE inhibitors is required. This work presents two new HNE inhibitor peptides, which were synthesized based on the reactive-site loop of the Bowman–Birk inhib...
Protein matrices for improved wound healing : elastase inhibition by a syntheti...
Vasconcelos, Andreia; Pêgo, A. P.; Lamghari, M.; Henriques, L.; Paulo, Artur CavacoThe unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman−Birk Inhibitor (BBI) protein. In vitro degradation and r...
Biodegradable materials based on silk fibroin and keratin
Vasconcelos, Andreia; Freddi, G.; Paulo, Artur CavacoWool and silk were dissolved and used for the preparation of blended films. Two systems are proposed: (1) blend films of silk fibroin and keratin aqueous solutions and (2) silk fibroin and keratin dissolved in formic acid. The FTIR spectra of pure films cast from aqueous solutions indicated that the keratin secondary structure mainly consists of alpha-helix and random coil conformations. The IR spectrum of pure...
New developments of enzymatic treatments on cellulosic fibers
Kim, Su-yeon; Zille, Andrea; Vasconcelos, Andreia; Paulo, Artur CavacoACS Symposium Series, Vol. 972 ; In this review, we focused on the recent and non-conventional enzyme bioconversions of cellulosic fibers. Cellulosic fibers are the largest market of textile industry and also represent the most successful market for enzymes base processes in the textile area. The new enzyme developments presented on this paper include the strength recovering in resin-crosslinked fabrics, phosp...
Enzymatic removal of cellulose from cotton/polyester fabric blends
Vasconcelos, Andreia; Paulo, Artur CavacoThe production of light-weight polyester fabrics from a polyester/cotton blended fabric, by means of the enzymatic removal of the cellulosic part of the material, was investigated. The removal of cotton from the blended fabric yielded more than 80% of insoluble microfibrillar material by the combined action of high beating effects and cellulase hydrolysis.Other major features of this enzymatic process for conve...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||