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Biology and molecular characterization of necroviruses
Félix, M. Rosário; Varanda, Carla M.R.; Clara, M. Ivone E.Three necroviruses, family Tombusviridae, are known to infect Olea europaea L., Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D), often asymptomatically. OLV-1 has a wide geographical distribution whereas that of OMMVand TNV-D is not known as their discovery is recent, dating from 2005 and 2009, respectively. OMMV has received special attention because of its ori...
Evidence of olive mild mosaic virus transmission by Olpidium brassicae
Varanda, Carla M. R.; Silva, Marta S. M. R.; Félix, M. Rosário; Clara, M. IvoneTransmission of three strains of OMMV by an Olpidium sp. was evaluated and compared. The three strains were 1) an OMMV wild type (WT) recovered from olive trees, 2) an OMMV variant (L11) obtained after 15 serial passages of single local lesions induced in Chenopodium murale plants, and 3) a construct OMMV/OMMVL11 in which the coat protein (CP) gene replaced that of the wild type. A single-sporangial culture der...
Complete nucleotide sequence of an Olive latent virus 1 isolate from olive trees
Félix, M. Rosário; Cardoso, Joana M.S.; Varanda, Carla M.R.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) is a necrovirus belonging to the familyTombusviridae. It is a small icosahedral plant virus, which encapsidates a single stranded positivesense RNA. This virus was first isolated from symptomless olive trees in Italy [7] and afterwards in Jordan and Portugal [10, 4]. OLV-1 was also isolated from symptomatic hosts, such as citrus trees in Turkey [11] and tulips in Japan [9]. Up to no...
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