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Genetic diversity of the coat protein of olive latent virus 1 isolates
Varanda, Carla; Nolasco, Gustavo; Clara, Maria Ivone; Félix, Maria RosárioThe CP gene variability among 21 olive latent virus 1 (OLV-1) isolates obtained from different hosts and locations and at different times was assessed. Amplicons obtained by RT-PCR were cloned, and at least 10 sequences from each isolate were analyzed and compared. OLV-1 sequences available in GenBank were included. The encoded CPs consisted of 270 amino acids, except those of isolates G1S and C7 (269 aa) and G...
A degenerate pair of primers for simultaneous detection of four alphaand betane...
Varanda, Carla; Cardoso, Joana M.S.; Oliveira, Mônica; Oliveira, Solange; Clara, Maria Ivone; Félix, Maria RosárioThe high infection levels due to Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) (alphanecrovirus) and Tobacco necrosis virus D (TNV-D) (betanecrovirus) in Portuguese olive orchards prompted us to develop a rapid PCR-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with European Union regulations. A pair of deg...
Biology and molecular characterization of necroviruses
Félix, M. Rosário; Varanda, Carla M.R.; Clara, M. Ivone E.Three necroviruses, family Tombusviridae, are known to infect Olea europaea L., Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D), often asymptomatically. OLV-1 has a wide geographical distribution whereas that of OMMVand TNV-D is not known as their discovery is recent, dating from 2005 and 2009, respectively. OMMV has received special attention because of its ori...
Specific amino acids of Olive mild mosaic virus coat protein are involved in tr...
Varanda, Carla; Félix, Maria R.; Soares, Cláudio M.; Oliveira, Solange; Clara, M. IvoneAbstract: Transmission of Olive mild mosaic virus (OMMV) is facilitated by Olpidium brassicae (Wor.) Dang. An OMMV mutant (OMMVL11) containing two changes in the coat protein (CP), asparagine to tyrosine at position 189 and alanine to threonine at position 216, has been shown not to be Olpidium brassicae-transmissible owing to inefficient attachment of virions to zoospores. In this study, these amino acid chang...
Evidence of olive mild mosaic virus transmission by Olpidium brassicae
Varanda, Carla M. R.; Silva, Marta S. M. R.; Félix, M. Rosário; Clara, M. IvoneTransmission of three strains of OMMV by an Olpidium sp. was evaluated and compared. The three strains were 1) an OMMV wild type (WT) recovered from olive trees, 2) an OMMV variant (L11) obtained after 15 serial passages of single local lesions induced in Chenopodium murale plants, and 3) a construct OMMV/OMMVL11 in which the coat protein (CP) gene replaced that of the wild type. A single-sporangial culture der...
Multiplex RT-PCR for detection and identification of three necroviruses that in...
Varanda, Carla; Cardoso, Joana M.S.; Félix, M. Rosário; Oliveira, Solange; Clara, M.IvoneAn optimized multiplex RT-PCR assay was developed to discriminate three necrovirus (Olive latent virus 1 (OLV-1), Tobacco necrosis virus D (TNV-D) and Olive mild mosaic virus (OMMV)) that infect olive trees. An olive orchard consisting of 54 trees of cv. "Galega vulgar" in the south of Portugal was surveyed. dsRNA fraction was used as template and revealed the 3 viruses, singly or in multiple infections, presen...
Complete nucleotide sequence of an Olive latent virus 1 isolate from olive trees
Félix, M. Rosário; Cardoso, Joana M.S.; Varanda, Carla M.R.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) is a necrovirus belonging to the familyTombusviridae. It is a small icosahedral plant virus, which encapsidates a single stranded positivesense RNA. This virus was first isolated from symptomless olive trees in Italy [7] and afterwards in Jordan and Portugal [10, 4]. OLV-1 was also isolated from symptomatic hosts, such as citrus trees in Turkey [11] and tulips in Japan [9]. Up to no...
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