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State and specific growth estimation in heterologous protein production by Pich...

The productivity of an industrial fermentation process involving a filamentous microbe is heavily dependent on the morphological form adopted by the organism. The development of systems capable of rapidly and accurately characterizing morphology within a given process represents a significant challenge, as the complex phenotypes that are manifested are not easily quantified. Conventional parameters employed in ...

Observer based estimation in heterologous protein production by Pichia pastoris

The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for both basic research and industrial use. In order to achieve high productivity, constant product quality, as well as to allow optimization and control of biotechnological processes, real-time monitoring of the key fermentation variables (biomass, substrate and products) is of major academic and industrial releva...

Calibration of a simplified model describing an urban waste water treatment pil...

THE DEVELOPMENT OF ENZYMATIC SENSORS FOR THE CONTINUOUS MONITORING OF GLUCOSE A...

Enzymatic sensors for the continuous measurement of glucose and sucrose, with the use of an enzymatic microreactor and a continuous flow dilution line for the medium, are studied in this work. The effects of the best conditions for medium and sampling flow through the sensor were analysed for a glucose concentration range from 0.05 to 0.2 g/l , which was possible to extend to a range from 5.0 to 120 g/l using a...