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Retrospective analysis of clinical yeast isolates in a hospital in the centre o...
Paulo, Cristiana; Mourão, Cristina; Veiga, Pedro M.; Marques, Joana M.; Rocha, Graça; Alves, Ana F.; Querol, Amparo; Meliço-Silvestre, António A.We conducted a four-year (2003-2006) retrospective study of yeasts recovered in a hospital laboratory in the centre of Portugal to evaluate the epidemiology of yeast infections. Clinical isolates and data were gathered from 751 patients corresponding to 906 episodes of yeast infection. The isolates were first identified using classical and commercial methods, routinely employed at the hospital laboratory. We th...
Sour rot-damaged grapes are sources of wine spoilage yeasts
Malfeito-Ferreira, Manuel; Loureiro, Virgilio; Barata, Andre; Gonzalez, Sara; Querol, AmparoMinireview ; Yeast species of sound and sour rot-damaged grapes were analysed during fermentation and grape ripening in the vineyard, using general and selective culture media. During 2003 and 2004 vintages, microvinifications were carried out with sound grapes to which different amounts of grapes with sour rot were added. The wine spoilage species Zygosaccharomyces bailii was only recovered during fermentatio...
Molecular typing of the yeast species Dekkera bruxellensis and Pichia guillierm...
Malfeito-Ferreira, Manuel; Loureiro, Virgilio; Martorell, Patricia; Barata, Andre; Fernandez-Espinar, M. Teresa; Querol, AmparoA total of 63 strains of Dekkera bruxellensis and 32 strains of Pichia guilliermondii isolated from wine related environments were identified by restriction analysis of the 5.8S-ITS region of the rDNA. These strains were subjected to intraspecific discrimination using mtDNA restriction and RAPD-PCR analysis. The isolates identified as D. bruxellensis yielded 3 different molecular patterns of mtDNA restriction u...
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