Encontrados 16 documentos, a visualizar página 1 de 2
In vitro blood flow in a rectangular PDMS microchannel: experimental observatio...
Lima, R.; Wada, Shigeo; Tanaka, S.; Takeda, Motohiro; Ishikawa, Takuji; Tsubota, Ken-ichi; Imai, Yohsuke; Yamaguchi, TakamiProgress in microfabricated technologies has attracted the attention of researchers in several areas, including microcirculation. Microfluidic devices are expected to provide powerful tools not only to better understand the biophysical behavior of blood flow in microvessels, but also for disease diagnosis. Such microfluidic devices for biomedical applications must be compatible with state-of-the-art flow measur...
Microscale flow dynamics of red blood cells in a circular microchannel
Lima, R.; Nakamura, M.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe blood flow dynamics in microcirculation depends strongly on the motion, deformation and interaction of RBCs within the microvessel. This paper presents the application of a confocal micro-PTV system to track RBCs through a circular polydimethysiloxane (PDMS) microchannel. This technique, consists of a spinning disk confocal microscope, high speed camera and a diode-pumped solid state (DPSS) laser combined w...
Measurement of multi-red blood cells interactions in blood flow by confocal mic...
Lima, R.; Ishikawa, Takuji; Fujiwara, Hiroki; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Matsuki, Noriaki; Wada, Shigeo; Yamaguchi, TakamiIn microcirculation the flow behavior of red blood cells (RBCs) plays a crucial role in many physiological and pathological phenomena. For instance, the interaction of RBCs in shear flow is believed to play an important role to the thrombogenesis process. Despite the relevance of this phenomenon on the blood mass transport, very little studies have been performed during the years, partly due to the absence of a...
In vitro confocal micro-PIV measurements of blood flow in a square microchannel:...
Lima, R.; Wada, Shigeo; Takeda, Motohiro; Tsubota, Ken-ichi; Yamaguchi, TakamiA confocal microparticle image velocimetry (micro-PIV) system was used to obtain detailed information on the velocity profiles for the flow of pure water (PW) and in vitro blood (haematocrit up to 17%) in a 100-μm-square microchannel. All the measurements were made in the middle plane of the microchannel at a constant flow rate and low Reynolds number (Re=0.025). The averaged ensemble velocity profiles were fou...
Velocity fields of blood flow in microchannels using a confocal micro-PIV system
Lima, R.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe in vitro experimental investigations provide an excellent approach to understand complex blood flow phenomena involved at a microscopic level. This paper emphasizes an emerging experimental technique capable to quantify the flow patterns inside microchannels with high spatial and temporal resolution. This technique, known as confocal micro-PIV, consists of a spinning disk confocal microscope, high speed cam...
Tracking red blood cells in a circular PDMS microchannel using a confocal micro...
Lima, R.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe blood flow in microcirculation is characterized mainly by the flow of red blood cells (RBCs), which may be normal or pathological. This paper presents the application of a confocal micro-P1V system lo track RBCs through a circular polydimelhysiloxane (PDMS) microchannel. This technique, consists o!’ a spinning disk confocal microscope, high speed camera and a diode-pumped solid stale (DPSS) laser combined w...
Measurement of erythrocyte motions in microchannels by using a confocal micro-P...
Lima, R.; Ishikawa, Takuji; Takeda, Motohiro; Tanaka, S.; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiDetailed knowledge on the motion of individual red blood cells (RBCs) flowing in microchannels is essential to provide a better understanding on the blood rheological properties and disorders in microvessels. Several studies on both individual and concentrated RBCs have already been performed in the past. However, all studies used conventional microscopes and also ghost cells to obtain visible trace RBCs throug...
Blood cell motions and interactions in microchannels
Lima, R.; Ishikawa, Takuji; Fujiwara, Hiroki; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiDetailed knowledge on the motions and interactions of individual blood cells flowing in microchannels is essential to provide a better understanding on the blood rheological properties and disorders in microvessels. This paper presents the ability of a confocal micro-PTV system to track red blood cells (RBCs) through a 100 μm circular glass microchannel. The technique consists of a spinning disk confocal micros...
Confocal micro-PIV measurements of three-dimensional profiles of cell suspensio...
Lima, R.; Wada, Shigeo; Tsubota, Ken-ichi; Yamaguchi, TakamiA detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a...
A particle method for blood flow simulation: application to flowing red blood c...
Tsubota, Ken-ichi; Wada, Shigeo; Kamada, H.; Kitagawa, Y.; Lima, R.; Yamaguchi, TakamiA new computer simulation using a particle method was proposed to analyze the microscopic behavior of blood flow. A simulation region, including plasma, red blood cells (RBCs) and platelets, was modeled by an assembly of discrete particles. The proposed method was applied to the motions and deformations of a single RBC and multiple RBCs, and the thrombogenesis caused by platelet aggregation. It is expected that...
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