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Purification of plasmid DNA from clarified and non-clarified Escherichia coli l...
Nunes, C. Caramelo; Gabriel, M. F.; Almeida, Paulo; Marcos, João Carlos; Tomaz, C. T.In this study, berenil was tested as a ligand, specifically to purify plasmids of different sizes pVAX1-LacZ (6.05 Kbp) and pCAMBIA-1303 (12.361 Kbp) from clarified Escherichia coli alkaline lysates. For this purpose, chromatographic experiments were performed using Sepharose derivatized with berenil. The results showed that both pDNA molecules are completely purified using lower amounts of salt in the eluent t...
A ten years analysis of cork oak (Quercus suber L.) afforestation projects in t...
Tomaz, C.; Monteiro, J.M.; Alegria, C.M.M.; Teixeira, M.C.C.Portuguese forest area has a great expression being around 39%. Since the last centuries the Quercus suber L.(cork oak) areas have increased, reaching to 715 922 ha (23%) according to the last National forest inventory. Afforestation programmes financed by the European Union and the existing protection laws for the species are one of the main reasons for this increase. These efforts are important to promote for...
Specific berenil–DNA interactions : an approach for separation of plasmid isofo...
Caramelo-Nunes, C.; Tente, T.; Almeida, P.; Marcos, João Carlos; Tomaz, C. T.Small molecules, like some antibiotics and anticancer agents that bind DNA with high specificity can represent a relevant alternative as ligands in affinity processes for plasmid DNA (pDNA) purification. In the present study, pDNA binding affinities of berberine, berenil, kanamycin and neomycin were evaluated by a competitive displacement assay with ethidium bromide using a fluorimetric titration technique. The...
Evaluation of five sugar cane planters
Rípoli,Marco L. C.; Rípoli,Tomaz C. C.Brazil is the world biggest producer of sugar cane with an area of 7x10(6) hectares. Mainly the system used for planting is the semi-mechanized one, which consists in opening the furrows with a machine, manually allocating the fractioned stalks and then covering the furrows done by the machines. The great amount of human labor used in the semi-mechanized system is becoming harder to find and also more expensive...
Evidence of hemispheric specialization in marmosets (Callithrix penicillata) us...
Tomaz,C.; Verburg,M.S.; Boere,V.; Pianta,T.F.; Belo,M.Recent studies have employed tympanic thermometry to assess lateralization of cognitive and emotional functions in primates. However, no studies using this technique have investigated the possibility of hemispheric specialization in New World monkeys. Therefore, the aim of the present study was to investigate tympanic membrane (TM) temperature asymmetries and their possible correlation with stress responses in ...
Enhancement of declarative memory associated with emotional content in a Brazil...
Frank,J.E.; Tomaz,C.Several studies have documented that emotional arousal may enhance long-term memory. This is an adaptation of a paradigm previously used in North American and European samples in investigations of the influence of emotion on long-term retention. A sample of 46 healthy adults of high and low educational levels watched a slide presentation of stories. A randomly assigned group watched a story with an arousing con...
Effects of emotional reactivity on inhibitory avoidance in the elevated T-maze
Conde,C.; Costa,V.; Tomaz,C.The possibility of the presence of inter-individual emotional differences and the memory performance of rats was examined in the elevated T-maze. Two kinds of aversively motivated behaviors, inhibitory avoidance and escape learning, were measured. Based on the number of trials to achieve a learning criterion, rats were divided into two subgroups with either low or high avoidance reactivity (LAR or HAR, respecti...
A Fault Tolerant Colored Petri Net Model for Flexible Manufacturing Systems
Barros,Tomaz C.; Figueiredo,Jorge C.A. de; Perkusich,AngeloThis paper introduces an approach based on Colored Petri Nets (CPN) to systematically introduce fault-tolerance in the design of a supervisor for a Flexible Manufacturing System (FMS). The system is modeled by means of Place/Transition nets and then is structurally reduced, resulting in a CPN that is independent of a specific production route. The introduction of fault tolerance in the design of such a supervis...
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