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Matrix Gla protein expression: a complex process involving the use of alternati...
Cancela, Leonor; Laizé, Vincent; Conceição, N.; Tiago, Daniel M.; Maia, A. T.; Bensimon-Brito, A.; Gavaia, Paulo J.Matrix Gla protein (MGP) is a secreted vitamin K-dependent protein (VKD) located in the extracellular matrix and capable of binding calcium through its -carboxyglutamate residues. Although identified in 1983, transcriptional and post-transcriptional mechanisms regulating its expression remain unclear.
Global analysis of gene expression in mineralizing fish vertebra-derived cell l...
Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, LeonorBackground Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic cell lines ...
Global analysis of gene expression in mineralizing fish vertebra-derived cell l...
Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, LeonorAbstract Background Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic ce...
Desenvolvimento de sistemas celulares de peixe adequados ao estudo da mineraliz...
Marques, C. L.; Rafael, M. S.; Tiago, Daniel M.; Cancela, Leonor; Laizé, VincentOs peixes foram recentemente reconhecidos como organismos modelo apropriados para o estudo da biologia de vertebrados, particularmente de processos relacionados com a mineralização tecidular e o desenvolvimento do esqueleto. Apesar de existirem alguns estudos in vivo, a identificação de mecanismos associados à calcificação em peixes tem sido prejudicada pelo facto de não existirem sistemas celulares para estudo...
Impairment of mineralization by metavanadate and decavanadate solutions in a fi...
Tiago, Daniel M.; Laizé, Vincent; Cancela, Leonor; Aureliano, M.Vanadium, a trace metal known to accumulate in bone and to mimic insulin, has been shown to regulate mammalian bone formation using in vitro and in vivo systems. In the present work, short- and long-term effects of metavanadate (containing monomeric, dimeric, tetrameric and pentameric vanadate species) and decavanadate (containing decameric vanadate species) solutions on the mineralization of a fish bone-derive...
Vanadate effects on bone metabolism: fish cell lines as an alternative to mamma...
Tiago, Daniel M.; Laizé, Vincent; Aureliano, M.; Cancela, LeonorVanadate, one of the most relevant forms of vanadium in solution, has been associated with the regulation of various enzyme activities (e.g. phosphatases, ribonucleases, ATPases, etc.) and shown to exhibit important biological effects. Several in vivo and in vitro studies have clearly demonstrated that any deficiency or excess of vanadium can seriously affect bone formation and its metabolism. Bone-related effe...
Vanadate and bone metabolism: effect on proliferation and mineralization of fis...
Tiago, Daniel M.; Laizé, Vincent; Cancela, Leonor; Aureliano, M.Vanadate is known for mimicking insulin action through activation of insulin and/or insulin like growth factor 1 (IGF 1) receptors. Vanadate insulin- like effect on bone-related metabolism has been previously investigated using mammalian in vitro cell systems but other vertebrate systems have rarely been used. We have recently demonstrated the suitability of a fish bone derived cell line (VSa13) to study anti-m...
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