Introduction. Exposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content in ambient air is unknown. HIALINE therefore monitored atmospheric concentrations of Betula, Poaceae and Olea pollen grains and matched their major allergen...
Pollen is routinely monitored, but it is unknown whether pollen counts represent allergen exposure. We therefore simultaneously determined olive pollen and Ole e 1 in ambient air in C ordoba, Spain, and Evora, Portugal, using Hirst-type traps for pollen and high-volume cascade impactors for allergen. Pollen from different days released 12-fold different amounts of Ole e 1 per pollen (both locations P < 0.001)....
The results in Italy should be compared with those from partners in other counlries. II will be important to confirm some observations during the first and second year of the projecl activity which showed the presence of a different allergenic power in difleren! geographical areas as observed for other types of pollen (Poaceae and Olea). The aim of lhe project was to measure the pollen's capacity to release all...
The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in SW Europe C. Antunes1*, R. Ferro1, R. Ribeiro1, Torres M.C.3, M.J. Velasco3H. García-Mozo3, Galán, C3 , R.Brandao2, M.Thibaudon4 and the HIALINE team5 1Department of Chemistry, University of Évora, Portugal 2Department of Biology, University of Évora, Portugal 3Department of Botany, Ecolog...
Exposure to allergens is one of severa1 factors determining sensitization and allergic symptoms in individuals. Exposure to aeroallergens from pollen is assessed by counting allergenic pollen in ambient air. However, proof is lacking that pollen count is representative for allergen exposure. We therefore monitored simultaneously birch, grass and olive pollen counts and their corresponding major pollen allergen...
airborn pollen quantification in outdoor air samples
Currently allergen exposure is estimated from pollen counts in air samples. However, there is not yet enough evidence to establish this technique as a reliable indicator of allergen exposure. Presently, there are a few reliable and sensitive ELISA methods that allow allergen quantification in environmental air samples but none is known to quantify Poaceae allergens. The aim of this work was to develop a novel ...
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