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Inducible nitric oxide synthase isoform is a key mediator of leukostasis and bl...
Leal, Ermelindo C.; Manivannan, Ayyakkannu; Hosoya, Ken-Ichi; Terasaki, Tetsuya; Cunha-Vaz, José; Ambrósio, António Francisco; Forrester, John V.PURPOSE: Nitric oxide (NO) is involved in leukostasis and blood-retinal barrier (BRB) breakdown in the early stages of diabetic retinopathy (DR), but it is unclear which NO synthase (NOS) isoforms are primarily involved. In this study, the authors aimed to clarify the involvement of constitutive (eNOS, nNOS) and inducible NOS (iNOS) isoforms and the mechanisms underlying NO-mediated leukostasis and BRB breakdow...
Downregulation of retinal GLUT1 in diabetes by ubiquitinylation
Fernandes, Rosa; Carvalho, Ana Luisa; Kumagai, Arno; Seica, Raquel; Hosoya, Ken-ichi; Terasaki, Tetsuya; Murta, Joaquim; Pereira, Paulo; Faro, CarlosPurpose: To investigate the effect of chronic hyperglycemia on the levels of the glucose transporter GLUT1 in retina and its ubiquitinylation. Methods: Two diabetic animal models (Goto Kakizaki rats and alloxan-induced diabetic rabbits) and retinal endothelial cells in culture were used. GLUT1 content was determined by western blotting. Glut1 mRNA was determined by RT-PCR and northern blotting. Ubiquitin conjug...
Downregulation of retinal GLUT1 in diabetes by ubiquitinylation
Fernandes, Rosa; Carvalho, Ana Luisa; Kumagai, Arno; Seica, Raquel; Hosoya, Ken-Ichi; Terasaki, Tetsuya; Murta, Joaquim; Pereira, Paulo; Faro, CarlosPurpose: To investigate the effect of chronic hyperglycemia on the levels of the glucose transporter GLUT1 in retina and its ubiquitinylation. Methods: Two diabetic animal models (Goto Kakizaki rats and alloxan-induced diabetic rabbits) and retinal endothelial cells in culture were used. GLUT1 content was determined by western blotting. Glut1 mRNA was determined by RT-PCR and northern blotting. Ubiquitin conjug...
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