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Blood protein adsorption onto chitosan
Benesch, Johan; Tengvall, P.Chitosan was recently indicated to enhance osteogenesis, improve wound healing but to activate the coagulation and the complement systems. In the present study approximately 10nm thick chitosan film were prepared on aminopropyltriethoxysilane (APTES) coated silicon. The surfaces were incubated in serum or plasma and subsequently in antibodies towards key complement and contact activation of coagulation proteins...
Protein adsorption to oligo (Ethylene Glycol) self assembled monolayers with am...
Benesch, Johan; Svedhem, S.; Svensson, S. C.; Valiokas, R.; Liedberg, B.; Tengvall, P.Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of brinogen, serum, and plasma were studied by ellipsometry on a series of well-de ned oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the g...
Quantification of adsorbed human serum albumin : a comparison between radioimmu...
Benesch, Johan; Askendal, A.; Tengvall, P.Radioimmunoassay (RIA) and null ellipsometry are two common methods to quantify adsorbed proteins. However, the accuracy of null ellipsometry with a constant protein refractive index (n 1.465, k 0) at l 632.8 nm has this far not been explored. The present study compared the methods, and the degree of agreement between the simplified single wavelength null ellipsometry and RIA to quantify adsorbed proteins was e...
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