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Screening of novel yeast inulinases and further application to bioprocesses
Paixão, Susana M.; Teixeira, Pedro D.; Silva, Tiago P.; Teixeira, A. V.; Alves, Luís ManuelInulin is a carbohydrate composed of linear chains of ß-2,1-linked D-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. Jerusalem artichoke (JA) is one of the most interesting materials among unconventional and renewable raw materials, with levels of inulin reaching 50–80% of dry matter. Inulin or inulin-rich materials can be actively hydrolyzed by micro...
Zygosaccharomyces bailii strain talf1 inulinases: a versatile tool for bioproce...
Paixão, Susana M.; Teixeira, Pedro D.; Silva, Tiago P.; Teixeira, A. V.; Alves, Luís ManuelFructans are one of the most abundant non-structural polysaccharides found in a wide range of plants. Inulin is a polydisperse fructan polymer composed by linear chains of b-2, 1-linked D-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. Inulin or inulin-rich materials can be actively hydrolyzed into fermentable sugars (glucose and fructose) using inuli...
Flow cytometric method for cell viability evaluation of Gordonia alkanivorans s...
Teixeira, A. V.; Silva, Tiago P.; Silva, Teresa Lopes da; Paixão, Susana M.; Alves, Luís ManuelThis work reports the development of a rapid flow cytometric method for the viability assessment of Gordonia alkanivorans strain 1B, a bacterium used in the biodesulfurization process. To demonstrate that it is possible to monitor by flow cytometric analysis changes in this bacterium physiological state, positive controls using the 5(6)-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining mixt...
Flow cytometric method for cell viability evaluation of Gordonia alkanivorans s...
Teixeira, A. V.; Silva, Tiago P.; Silva, Teresa Lopes da; Paixão, Susana M.; Alves, Luís ManuelThe most commonly method used for sulfur removal from fossil fuels is hydrodesulfurization, a physico-chemical process at very high temperatures and pressures. An alternative to this process is biodedesulfurization (BDS), a microbiological process that works at atmospheric pressure and temperature making it easier to work with and less expensive. It also as the advantage of easily desulfurizing recalcitrant sul...
Optimization of low sulfur carob pulp liquor as carbon source for fossil fuels ...
Silva, Tiago P.; Paixão, Susana M.; Teixeira, A. V.; Roseiro, J. Carlos; Alves, Luís ManuelBackground:Biodesulfurization (BDS) is a complementary technology to hydrodesulfurization since it allows the removal of recalcitrant sulfur compounds present in fossil fuels. The cost of culture medium to produce the biocatalysts is still one limitation for BDS application. Carob pulp, as an alternative carbon source, can reduce this cost. However, the presence of sulfates is critical, since BDS is inhibited a...
Helicobacter pylori, nonsteroidal anti-inflammatory agents and gastroduodenal c...
Teixeira, A V; Serviço de Medicina 1, Hospital de S. João, Faculdade de Medicina do Porto.The author discusses the possible interactions between non-steroidal anti-inflammatory drugs (NSAIDs) and Helicobacter pylori (Hp) which may play an important role in the unleashing of gastroduodenal lesions. To our knowledge, AINEs have no influence on the prevalence of infection by Hp and the latter does not seem to influence the development and intensity of the lesions caused by NSAIDs. ; The author discuss...
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