Encontrados 4 documentos, a visualizar página 1 de 1
Contribution of coagulant and native microflora to the volatile-free fatty acid...
Tavaria, Freni K.; Tavares, Tânia G; Ferreira, A.C.Silva; Malcata, F. XavierThe contributions of the coagulant Cynara cardunculus and of the microflora of raw milk to the volatile-free fatty acid profile of Serra da Estrela cheese were evaluated. The experimental design included both a model system and, dual cheeses. The study in the model system showed that isovaleric acid was the predominant volatile compound after 7 d of ripening. The systems inoculated with Enterococcus faecium pro...
Influence of native lactic acid bacteria on the microbiological, biochemical an...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierCheesemaking from batches of raw ewe’s milk was carried out via inoculation with wild strains of Lactococcus lactis subsp. lactis ESB110019 and Lactobacillus plantarum ESB5004 independently, or combined with each other. Those two strains had been isolated from the native microflora of typical Serra da Estrela cheese. One control batch was processed in parallel without addition of any starter. The evolution in v...
Esterase activities of intracellular extracts of wild strains of lactic acid ba...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierLactococcus lactis subsp. lactis strain ESB110019 and Lactobacillus plantarum strain ESB5004, novel strains that were previously isolated from the wild adventitious microflora of certified Serra da Estrela cheeses, were assayed for esterase activity using, as substrates, ortho- and para-nitrophenyl derivatives of fatty acids. Both strains preferentially hydrolyzed short-chain fatty acids; L. lactis ESB110019 ex...
Purification and characterization of an intracellular aminopeptidase from a wil...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierAn intracellular hydrolase able to cleave Image -lysine-p-nitroanilide was purified from Lactobacillus plantarum strain ESB5004 via two steps of precipitation with ammonium sulfate (at 30 and 50% (w/v)), followed by hydrophobic interaction and ion-exchange chromatographies. The aminopeptidase was purified up to 11-fold, with a final yield of ca. 1%. Its native molecular weight is ca. 70 kDa, and it is apparentl...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||