This work was supported by the Portuguese National Science Foundation (FCT) through a grant attributed to the CNC Institution (PEst-C/ SAU/LA0001/2011) and a Wellcome Trust Grant #86470 provided to S.J.P. and C.L.R.B. S.M. was supported by the Infertility Research Trust. R.S.T. is the recipient of a PhD fellowship from FCT (SFRH/ BD/46002/2008).
http://www.sciencedirect.com/science/article/B6TC0-4TRCYC3-1/2/23f0cc8f01dd94bc8f398c89b9855d73
BACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
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