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Laccase recovery with aqueous two-phase systems : enzyme partitioning and stabi...
Silvério, Sara C.; Rodríguez, Oscar; Tavares, Ana P. M.; Teixeira, J. A.; Macedo, EugéniaIn this work, the potential of several aqueoustwo-phase systems (ATPS) for laccaserecovery was evaluated. For this purpose, different types of ATPS were prepared and the partition coefficient of pure commercial laccase was determined in each one, at 23 °C. A total of 21 ATPS were investigated: 12 composed by a polymer and a salt and 9 composed by two different polymers. For polymer-salt ATPS, different composit...
Immobilization of commercial laccase on spent grain
Silva, Andreia Machado da; Tavares, Ana P. M.; Rocha, Cristina M. R.; Cristóvão, Raquel O.; Teixeira, J. A.; Macedo, Eugénia A.The aim of this work was to assess the possibility of using beer spent grain (a byproduct of beer’s brewing industry) as a carrier for laccase immobilization. Both adsorption (on spent grain – SG and on digested spent grain – DSG) and covalent binding (using glycidol and glycidol followed by ethylenediamine on DSG) were used. The effect of different immobilization conditions on the immobilization yields and rec...
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