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Axisymmetric PDMS (polydimethysiloxane) microchannels for in vitro haemodynami...
Lima, R.; Oliveira, M.S.N.; Ishikawa, Takuji; Kaji, H.; Tanaka, S.; Nishizawa, Matsuhiko; Yamaguchi, TakamiThe current microdevices used for biomedical research are often manufactured using microelectromechanical systems (MEMS) technology. Although it is possible to fabricate precise and reproducible rectangular microchannels using soft lithography techniques, this kind of geometry may not reflect the actual physiology of the microcirculation. Here, we present a simple method to fabricate circular PDMS microchannels...
In vitro blood flow in a rectangular PDMS microchannel: experimental observatio...
Lima, R.; Wada, Shigeo; Tanaka, S.; Takeda, Motohiro; Ishikawa, Takuji; Tsubota, Ken-ichi; Imai, Yohsuke; Yamaguchi, TakamiProgress in microfabricated technologies has attracted the attention of researchers in several areas, including microcirculation. Microfluidic devices are expected to provide powerful tools not only to better understand the biophysical behavior of blood flow in microvessels, but also for disease diagnosis. Such microfluidic devices for biomedical applications must be compatible with state-of-the-art flow measur...
Microscale flow dynamics of red blood cells in a circular microchannel
Lima, R.; Nakamura, M.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe blood flow dynamics in microcirculation depends strongly on the motion, deformation and interaction of RBCs within the microvessel. This paper presents the application of a confocal micro-PTV system to track RBCs through a circular polydimethysiloxane (PDMS) microchannel. This technique, consists of a spinning disk confocal microscope, high speed camera and a diode-pumped solid state (DPSS) laser combined w...
Velocity fields of blood flow in microchannels using a confocal micro-PIV system
Lima, R.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe in vitro experimental investigations provide an excellent approach to understand complex blood flow phenomena involved at a microscopic level. This paper emphasizes an emerging experimental technique capable to quantify the flow patterns inside microchannels with high spatial and temporal resolution. This technique, known as confocal micro-PIV, consists of a spinning disk confocal microscope, high speed cam...
Tracking red blood cells in a circular PDMS microchannel using a confocal micro...
Lima, R.; Ishikawa, Takuji; Tanaka, S.; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiThe blood flow in microcirculation is characterized mainly by the flow of red blood cells (RBCs), which may be normal or pathological. This paper presents the application of a confocal micro-P1V system lo track RBCs through a circular polydimelhysiloxane (PDMS) microchannel. This technique, consists o!’ a spinning disk confocal microscope, high speed camera and a diode-pumped solid stale (DPSS) laser combined w...
Measurement of erythrocyte motions in microchannels by using a confocal micro-P...
Lima, R.; Ishikawa, Takuji; Takeda, Motohiro; Tanaka, S.; Imai, Yohsuke; Tsubota, Ken-ichi; Wada, Shigeo; Yamaguchi, TakamiDetailed knowledge on the motion of individual red blood cells (RBCs) flowing in microchannels is essential to provide a better understanding on the blood rheological properties and disorders in microvessels. Several studies on both individual and concentrated RBCs have already been performed in the past. However, all studies used conventional microscopes and also ghost cells to obtain visible trace RBCs throug...
Velocity measurements of blood flow in a rectangular PDMS microchannel assessed...
Lima, R.; Wada, Shigeo; Tanaka, S.; Takeda, Motohiro; Tsubota, Ken-ichi; Ishikawa, Takuji; Yamaguchi, TakamiThis paper examines the ability to measure the velocity of both physiological saline (PS) and in vitro blood in a rectangular polydimethysiloxane (PDMS) microchannel by means of the confocal micro-PIV system. The PDMS microchannel, was fabricated by conventional soft lithography, had a microchannel near to a perfect rectangular shape (300μm wide, 45μm deep) and was optically transparent, which is suitable to me...
Determination of serum aluminum, platelet aggregation and lipid peroxidation in...
Neiva,T.J.C.; Benedetti,A.L.; Tanaka,S.M.C.N.; Santos,J.I.; D'Amico,E.A.Aluminum (Al3+) overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment...
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