The aim of this work was to investigate the effect of phenolic acids on cell membrane permeability of lactic acid bacteria from wine. Several phenolic acids were tested for their effects on the cell membrane of Oenococcus oeni and Lactobacillus hilgardii by measuring potassium and phosphate efflux, proton influx and by assessing culture viability employing a fluorescence technique based on membrane integrity. T...
Flow injection analysis (FIA) methods for the spectrophotometric determination of CO2 and SO2 in wines are described. The determination of CO2 is based on the colour change of a low capacity buffer (containing an acid-base indicator) due to the dissolved carbon dioxide. The determination of SO2 is based on the decoloration of malachite green by sulphur dioxide. Two FIA manifolds are presented; one for the deter...
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