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Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) ...
Franklin, Gregory; Arvinth, S.; Sheeba, Caroline J.; Kanchana, M.; Subramonian, N.We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explant...
Data: 2006
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Origem: RepositóriUM - Universidade do Minho
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