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Isolation of an acetyl-CoA synthetase gene (ZbACS2) from Zygosaccharomyces bailii
Rodrigues, Fernando José dos Santos; Zeeman, Anne-Marie; Cardoso, M. Helena; Sousa, Maria João; Steensma, H. Yde; Leão, CecíliaA gene homologous to Saccharomyces cerevisiae ACS genes, coding for acetyl-CoA synthetase, has been cloned from the yeast Zygosaccharomyces bailii ISA 1307, by using reverse genetic approaches. A probe obtained by PCR amplification from Z. bailii DNA, using primers derived from two conserved regions of yeast ACS proteins, RIGAIHSVVF (ScAcs1p; 210–219) and RVDDVVNVSG (ScAcs1p; 574–583), was used for screening a ...
The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening ...
Rodrigues, Fernando José dos Santos; Ludovico, Paula; Sousa, Maria João; Steensma, H. Yde; Côrte-Real, Manuela; Leão, CecíliaZygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of...
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