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Artefacts induced on c-type haem proteins by electrode surfaces
Sousa, Patrícia M. Paes de; Pauleta, Sofia R.; Gonçalves, M. Lurdes Simões; Pettigrew, Graham W.; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2011) 16:209–215 DOI 10.1007/s00775-010-0717-z ; In this work it is demonstrated that the characterization of c-type haem containing proteins by electrochemical techniques needs to be cautiously performed when using pyrolytic graphite electrodes. An altered form of the cytochromes, which has a redox potential 300 mV lower than that of the native state and displays peroxidatic activity, can b...
A new CuZ active form in the catalytic reduction of N2O by nitrous oxide reduct...
Dell’Acqua, Simone; Pauleta, Sofia R.; Sousa, Patrícia M. Paes de; Monzani, Enrico; Casella, Luigi; Moura, José J. G.; Moura, IsabelJ Biol Inorg Chem (2010) 15:967–976 DOI 10.1007/s00775-010-0658-6 ; The final step of bacterial denitrification, the two-electron reduction of N2O to N2, is catalyzed by a multi-copper enzyme named nitrous oxide reductase. The catalytic centre of this enzyme is a tetranuclear copper site called CuZ, unique in biological systems. The in vitro reconstruction of the activity requires a slow activation in the pres...
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