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Activity of Thymus capitellatus volatile extract, 1,8-cineoleand borneol agains...

In the search for new leishmanicidal agents, Thymus capitellatus Hoffmanns. & Link (familyLamiaceae) volatile extract and its major compounds, 1,8-cineole and borneol, were testedagainst Leishmania infantum, Leishmania tropica and Leishmania major. Plant volatile extract(essential oil) was analysed by GC and GC–MS and the activity of essential oil on Leishmaniapromastigotes viability was assessed using tetrazol...

Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol again...

In the search for new leishmanicidal agents, Thymus capitellatus Hoffmanns. & Link (family Lamiaceae) volatile extract and its major compounds, 1,8-cineole and borneol, were tested against Leishmania infantum, Leishmania tropica and Leishmania major. Plant volatile extract (essential oil) was analysed by GC and GC–MS and the activity of essential oil on Leishmania promastigotes viability was assessed using tetr...

A PRND polymorphism in Churra do Campo portuguese sheep breed

Prion-like Doppel gene (PRND) is located downstream from priori protein gene (PRNP). Doppel protein is not related to prion disease but to male fertility. Our previous analysis of PRND coding region in 460 animals from 8 Portuguese sheep breeds, by multiple restriction fragment-single strand conformation polymorphism (MRFSSCP), revealed a synonymous substitution (78G>A). An association was found between identif...

Análise da estrutura genética de populações ovinas churras portuguesas

A diversidade e estrutura genética foram estudadas em seis raças portuguesas do grupo Churro (Badana, Galega Bragançana, Galega Mirandesa, Mondegueira, Churra da Terra Quente e Algarvia), e na raça exótica Assaf, com um conjunto de 20 microssatélites. Os dados foram analisados com a metodologia bayesiana implementada pelo software STRUCTURE. A variabilidade genética observada sugere a existência de quatro popul...

Genetic diversity in native and commercial breeds of pigs in Portugal assessed ...

Population structure and genetic diversity in the Portuguese native breeds of pigs Alentejano (AL), Bísaro (BI), and Malhado de Alcobaça (MA) and the exotic breeds Duroc (DU), Landrace (LR), Large White (LW), and Pietrain were analyzed by typing 22 microsatellite markers in 249 individuals. In general, the markers used were greatly polymorphic, with mean total and effective number of alleles per locus of 10.68 ...

Avaliação das Concentrações Pré-industriais e Actuais de Ozono Superficial atra...

The cytotoxic effects of ciprofloxacin in Giardia lamblia trophozoites

The cytotoxicity of ciprofloxacin was investigated in culture of Giardia lamblia (ATCC 30957), used as an in vitro cellular model, on the basis of cell growth, morphology, viability, adherence and metabolic studies. The effects on cell membrane integrity were evaluated by permeability of the cells to trypan blue, and the morphological alterations were studied by optical and transmission electronic microscopy. T...

Cytotoxicity Induced by Bismuth Subcitrate in Giardia lamblia Trophozoites

The cytotoxicity of colloidal bismuth subcitrate (CBS) was investigated in cultured Giardia lamblia trophozoites on the basis of cell attachment, morphology and viability studies. The effects on cell membrane integrity were evaluated by the permeability to trypan blue, and the morphological alterations were studied by phase-contrast microscopy and transmission electron microscopy. Our data show that although CB...