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Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sper...
Sousa, Ana Paula M.; Tavares, Renata S.; Velez de la Calle, Juan Felipe; Figueiredo, Helena; Almeida, Vasco; Almeida-Santos, TeresaBACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
Characterization of human sperm populations using conventional parameters, surf...
Varum, Sandra; Bento, Carla; Sousa, Ana Paula M.; Gomes-Santos, Carina S. S.; Henriques, Paula; Almeida-Santos, Teresa; Teodósio, Cristina; Paiva, ArturTo directly compare distinct assays proposed to monitor human sperm quality and possibly preselect sperm populations for assisted reproductive technology (ART).Design ; http://www.sciencedirect.com/science/article/B6T6K-4MD46F2-4/1/881ded71ecdcac2b19dc8841945d9b66
Teaching about citric acid cycle using plant mitochondrial preparations: Some a...
Vicente, Joaquim A. F.; Gomes-Santos, Carina S. S.; Sousa, Ana Paula M.; Madeira, Vítor M. C.Potato tubers and turnip roots were used to prepare purified mitochondria for laboratory practical work in the teaching of the citric acid cycle (TCA cycle). Plant mitochondria are particularly advantageous over the animal fractions to demonstrate the TCA cycle enzymatic steps, by using simple techniques to measure O2 consumption and transmembrane potential (ΔΨ). The several TCA cycle intermediates induce speci...
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