The human ejaculate contains subpopulations of sperm with distinct properties. Human X- and Y-bearing sperm were separated with fluorescence activated cell sorting. To avoid the use of UV light the quantitative DNA dyes DRAQ5® and Dyecycle™ Vybrant® Violet were used. Sorting efficiency was similar for both dyes, but lower than what is usually obtained with the classical method involving Hoechst 33342 and UV lig...
Tese de mestrado. Fundamentos e Aplicações da Mecânica de Fluidos. 2006. Faculdade de Engenharia. Universidade do Porto
Tese de mestrado em Engenharia Informática, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2012 ; Há em Portugal cerca de 30 mil pessoas que têm como primeira língua, a Língua Gestual Portuguesa (LGP). O Kinect é uma ferramenta de custo acessível que pode ser usado para o reconhecimento e tradução de gestos desta língua para a Língua Portuguesa, usando uma aplicação adequada. Para o re...
Human sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obta...
A educação especial e inclusão social da criança diferente em espaço de recreio comum, numa escola regular, embora desejável, na realidade não acontece. De facto, a prática docente dos últimos anos permite-nos afirmar que a teoria, relativamente a este assunto, é sempre pensada e registada no projecto educativo da escola. Posteriormente, quando se passa à sua aplicação prática, verificamos que não é possível re...
BACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
To directly compare distinct assays proposed to monitor human sperm quality and possibly preselect sperm populations for assisted reproductive technology (ART).Design ; http://www.sciencedirect.com/science/article/B6T6K-4MD46F2-4/1/881ded71ecdcac2b19dc8841945d9b66
Potato tubers and turnip roots were used to prepare purified mitochondria for laboratory practical work in the teaching of the citric acid cycle (TCA cycle). Plant mitochondria are particularly advantageous over the animal fractions to demonstrate the TCA cycle enzymatic steps, by using simple techniques to measure O2 consumption and transmembrane potential (ΔΨ). The several TCA cycle intermediates induce speci...
In this work highly dispersed Ru-Sn bimetallic catalysts have been prepared from organobimetallic Cp(PPh3)2Ru-SnX3 (X = Cl or Br) complexes. These single source precursors can be easily impregnated in high surface area supports, such as activated carbon and sol-gel SiO2, and upon controlled thermal treatment the ligands are released as volatile products resulting in the formation of the bimetallic system Ru-Sn....
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