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A new approach to bacterial colony morphotyping by matrix-assisted laser desorp...

Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification ...

Small colony variants in biofilm population is not strictly required for biofil...

Pseudomonas aeruginosa is a well-known opportunistic pathogen, responsible for high mortality in several human infections. Such infection success is partly due to its phenotypic plasticity to switch to “fitter” phenotypes, such as small colony variants (SCV), generally associated to increased antibiotic resistance, and biofilm formation within the human body. Once embedded in biofilms, P. aeruginosa can augment...

MorphoCol : a powerful tool for the clinical profiling of pathogenic bacteria

Pathogenicity, virulence and resistance of infection-causing bacteria are noteworthy problems in clinical settings, even after disinfection practices and antibiotic courses. Although it is common knowledge that these traits are associated to phenotypic and genetic variations, recent studies indicate that colony morphology variations are a sign of increased bacterial resistance to antimicrobial agents (i.e. anti...

Computational approaches to standard-compliant biofilm data for reliable analys...

The study of microorganism consortia, also known as biofilms, is associated to a number of applications in biotechnology, ecotechnology and clinical domains. Nowadays, biofilm studies are heterogeneous and data-intensive, encompassing different levels of analysis. Computational modelling of biofilm studies has become thus a requirement to make sense of these vast and ever-expanding biofilm data volumes. The rat...

Adaptive response of single and binary Pseudomonas aeruginosa and Escherichia c...

The main goal of this work was to examine whether the continuous exposure of single and binary P. aeruginosa and E. coli biofilms to sub-lethal benzalkonium chloride (BC) doses can induce adaptive response of bacteria. Biofilms were formed during 24 h and then put continuously in contact with BC for more 5 days. The six-day-old adapted biofilms were then submitted to BC challenge, characterized and inspected by...

Phenotypic switching : an opportunity to bacteria thrive

Bacteria in nature are “plagued“ by various unpredictable environmental stresses, being population diversity one of the strategies adopted to survive. Phenotypic switching is one of the evolution processes that causes commutation between phenotypic states. This phenomenon shows up as variation in colony morphology. Alterations in colony morphotype traits may reveal altered cellular basis phenotype which can con...

Detection of resistant mutants within Pseudomonas aeruginosa colony morphology ...

Bacterial infections caused mainly by P. aeruginosa are typical of cystic fibrosis (CF) lung disease. Despite the long and aggressive antibiotic therapy, CF patients still died because of these chronic infections. The deprived bacterial eradication is mainly due to several strategies adopted by bacteria to achieve CF airways adaptation and tolerance to antibiotics. Biofilm formation and phenotypic switching are...

Prophylactic outcomes of casbane diterpene in Candida albicans and Candida glab...

Biofilms are surface associated communities of microorganisms embedded within a self-produced extracellular matrix and adhered on inert and biotic surfaces. These biological consortia are considered the most prevalent growth form of microorganisms. Biofilm formation is a potent virulence factor for a number of Candida species, as it confers significant tolerance to antimicrobial therapy, primarily by limiting t...

Casbane diterpene activity on mixed biofilms of P. aeruginosa and Candida species

Profiling of pathogenic bacteria by colony morphology – identification of poten...

Colony morphology changes may be an indicator of the phenotypic variations associated to the pathogenecity, virulence and antimicrobial resistance of infection-causing microorganisms. Particularly, phenotypic changes derived from biofilm growth and in response to environmental stressors. For instance, in patients with cystic fibrosis, P. aeruginosa colony morphology undergoes a conversion from non- to mucoi...