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Alginate coated chitosan nanoparticles are an effective subcutaneous adjuvant f...
Borges, Olga; Silva, Marta; Sousa, Adriano de; Borchard, Gerrit; Junginger, Hans E.; Cordeiro-da-Silva, Anabelahttp://www.sciencedirect.com/science/article/B6W7N-4TFV6D6-1/2/da901d7d04c884f0b04f835c6e86c774
Immune response by nasal delivery of hepatitis B surface antigen and codelivery...
Borges, Olga; Cordeiro-da-Silva, Anabela; Tavares, Joana; Santarém, Nuno; Sousa, Adriano de; Borchard, Gerrit; Junginger, Hans E.Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied b...
Evaluation of the immune response following a short oral vaccination schedule w...
Borges, Olga; Tavares, Joana; Sousa, Adriano de; Borchard, Gerrit; Junginger, Hans E.; Cordeiro-da-Silva, AnabelaThe purpose of this work was to assess the ability of recombinant hepatitis B vaccine, encapsulated in alginate-coated chitosan nanoparticles, to induce local and systemic immune responses following oral vaccination. The antigen was administered either alone or in combination with the immunopotentiator, synthetic oligodeoxynucleotide containing immunostimulatory CpG motif (CpG ODN) as adjuvant, and associated o...
Induction of lymphocytes activated marker CD69 following exposure to chitosan a...
Borges, Olga; Borchard, Gerrit; Sousa, Adriano de; Junginger, Hans E.; Cordeiro-da-Silva, AnabelaCD69 is a very early cell activation antigen expressed on the surface of activated immune cells. It can appear within 1-2 h of activation and exhibits maximal expression levels between 18 and 24 h after stimulation. In this work, the expression profile of CD69 in mice splenocytes was evaluated following exposure to the biopolymers, chitosan or alginate and the immunostimulatory factors, CpG ODN 1826 or concanav...
Uptake studies in rat Peyer's patches, cytotoxicity and release studies of algi...
Borges, Olga; Cordeiro-da-Silva, Anabela; Romeijn, Stefan G.; Amidi, Maryam; Sousa, Adriano de; Borchard, Gerrit; Junginger, Hans E.The design of particulate vaccine delivery systems, particularly for mucosal surfaces, has been a focus of interest in recent years. In this context, we have previously described the development and the characterization of a new nanosized delivery system, consisting of a model antigen adsorbed to chitosan particles and coated with sodium alginate. In the present work the ovalbumin release profiles from these co...
Preparation of coated nanoparticles for a new mucosal vaccine delivery system
Borges, Olga; Borchard, Gerrit; Verhoef, J. Coos; Sousa, Adriano de; Junginger, Hans E.It has been found that the adsorption of antigens onto chitosan particles is an easy and unique mild loading process suitable to be used with vaccines. In order to increase the stability of this particles and to prevent an immediate desorption in gastrointestinal fluids, a coating process with sodium alginate was developed. One of the challenges of this developing process was to keep the particles in the nanosi...
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