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Microbial conversion of glycerol to 1,3-propanediol$ephysiological comparison o...
González-Pajuelo, María; Meynial-Salles, Isabelle; Mendes, Filipa; Soucaille, Philippe; Vasconcelos, IsabelClostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum DG1, growth on glycerol was achieved, and 1,3-propanediol was produced. In order to compare th...
Metabolic engineering of clostridium acetobutylicum for the industrial producti...
González-Pajuelo, María; Meynial-Salles, Isabelle; Mendes, Filipa; Andrade, Jose Carlos; Vasconcelos, Isabel; Soucaille, PhilippeClostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. However, to develop an economical process of 1,3-propanediol production, it would be necessary to improve the strain by a metabolic engineering approach. Unfortunately, no genetic tools are currently available for C...
Regulation of metabolic shifts in Clostridium acetobutylicum ATCC 824
Girbala, Laurence; Crouxa, Christian; Vasconcelos, Isabel; Soucaille, PhilippeAlcohol formation was initiated in continuous cultures of Clostridium acetobutylicum under distinct steady-state conditions: (i) in glucose-limited cultures established at low operating pH with formation of butanol, ethanol and acetone (induction of the solventogenesis) in which cells contained normal levels of NADH and a high level of ATP and butyric acid; and (ii) by increasing the NADH pressure at neutral pH...
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